羟乙酸磺酸戊烷脒(PI)对人精子活动能力和体外受精能力的影响

羟乙酸磺酸戊烷脒(PI)对人精子活动能力和体外受精能力的影响

王英, 张树成, 邱劲松, 戴景峰, 孙亦彬, 张莹, 杨达志

1. 国家计生委科研所, 北京 100081;
2. 首都医科大学, 北京 100053

收稿日期:1993-03-03 修回日期:1993-07-16 出版日期:1994-12-15 发布日期:1994-12-15

Effect of Pentamidine Isethionate (PI) on Fertilizing Ability of Human Spermatozoa in vitro

Ying Wang, Shu-Cheng Zhang, Jin-Song Qiu, Jing-Feng Dai, Yi-Bin Sun, Ying Zhang, Da-zhi Yang

1. National Research Institute for Family Planning, Beijing 100081
2. Capital Medical University, Beijing 100053

Received:1993-03-03 Revised:1993-07-16 Online:1994-12-15 Published:1994-12-15

摘要/Abstract

摘要： 本文对人精子活率, 向前运动能力及穿透地鼠卵的能力在不同浓度(0, 0.5, 1.0和2.0 mg/ml)的PI作用下的改变情况进行了观察。结果表明随PI浓度递增及作用时间延长, 精子活率(M), III级向前运动精子的百分率明显降低(P<0.05)。并且对时间的依赖性和剂量依赖性有很好的负相关(P<0.05); 受精率, 受精指数和精卵亲合率明显下降(P<0.0l); 在PI作用下受精卵无原核形成的能力。提示PI具有抑制人精子生育能力的作用, 且作用可能是多环节的。

关键词: 羟乙酸磺酸戊烷脒, 人精子, 活动能力, 受精能力

Abstract: Various trations of PI(0, 0.5, 1.0and2.0mg/ml) were incubated with super-activated human spermatozoa at37 ºC for 5, 15 and 30min, then the vitality and motility of spermatozoa were examined, respectively, after which the washed spermatozoa were cultured with zone-free superovulated hamster ova for 3h. Observations were made for the presence of fertilized ova, ova attached-spermatozoa and pronuclei. The results showed that the vitality and III grade forward motility of spermatozoa were significantly reduced (P<0.05) after drug treament. There was a good negative relativity between vitality, motility and time, dose of PI treatment (P<0.05). The fertilization rate and mean attached spermatozoa/ova, the index of fertilization, were significantly reduced (P<0.01) as compared with controls. There was no pronuclei formation in the drug treated groups. It is suggested that PI may inhibit the ability of fertilization of human spermatozoa and the effect is expressed in several aspects.

Key words: Pentamidine Isethionate (PI), Human spermatozoa, Motility ability, Fertilizing ability

Supporting:

王英, 张树成, 邱劲松, 戴景峰, 孙亦彬, 张莹, 杨达志. 羟乙酸磺酸戊烷脒(PI)对人精子活动能力和体外受精能力的影响[J]. .

Ying Wang, Shu-Cheng Zhang, Jin-Song Qiu, Jing-Feng Dai, Yi-Bin Sun, Ying Zhang, Da-zhi Yang. Effect of Pentamidine Isethionate (PI) on Fertilizing Ability of Human Spermatozoa in vitro[J]. .