有机锡化合物抗肿瘤作用的研究

有机锡化合物抗肿瘤作用的研究

肖军军, 崔景荣, 苏雅娴, 贺杰, 姚均恩

1. 北京医科大学细胞生物学教研室, 北京100083;
2. 北京医科大学药学院生化药理研究室, 北京100083;
3. 中国科学院北京电子显微镜实验室, 北京100081

收稿日期:1992-03-18 修回日期:1993-02-23 出版日期:1993-06-15 发布日期:1993-06-15

Investigation on the Antitumor Activity of Organotin Compound

Jun-Jun Xiao, Jing-Rong Cui, Ya-Xian Su, Jie He, Jun-En Yao

1. Department of cell Biology.Beijing Medical University, Bejing 100083
2. Research Laboratory of Biochemical Pharmacology.School of Pharmaceutical Science Beijing Medical University.Beijing 100083
3. Beijing Laboratory of Electron Microscope.Academia Smica.Beijing 100081

Received:1992-03-18 Revised:1993-02-23 Online:1993-06-15 Published:1993-06-15

摘要/Abstract

摘要：

利用扫描电镜、描隧道显微镜及细胞荧光光度计, 研究了有机锡化合物Et2SnCl2phen 对人胃癌细胞(BGC-823)膜表面结构、膜蛋白和核中DNA 含量的影响。发现Et2SnCl2phen不但能抑制细胞生长, 而且能明显改变癌细胞膜表面结构。电镜下显示出癌细胞表面比较光滑和较少微绒毛。扫描隧道显微镜的图像显示出细胞表面不平和疏松分布。与对照组相比, 经Et2SnCl2phen 处理的细胞膜蛋白质和核中DNA 的含量明显减少。

关键词: 有机锡化合物(Et2SnCl2phen), 膜的表面结构, 膜蛋白, 核 DNA, 人胃癌细胞

Abstract:

Changes in the surface structure of cell membrane and the contents of membrane proteins and nuclear DNA of human gastric cancer (BGC-823) cells treated with organotin compound (Et2SnCl2phen) were studied with a scanning electron microscope (SEM), a scanning tunneling, microscope (STM), and a cytofluorophotometer. It was found that Et2SnCl2Phen not only inhibited the cell growth but also remarkably changed the surface structure of the membrane of cancer cells. The surface of Et2SnCl2phen treated cancer cells was relatively smooth and showed fewer microvilli under SEM. STM images showed an uneven and loose distribution of the surface of the cell. In comparison with the untreated cancer cells, there was an evident decrease in the content of membrane proteins and nuclear DNA in Et_2SnCl2phen treated cells.

Key words: Organotin compound (Et2SnCl2phen), Surface structure of membrane, Membrane proteins, Nuclear DNA, Human gastric cancer cells

Supporting:

肖军军, 崔景荣, 苏雅娴, 贺杰, 姚均恩

. 有机锡化合物抗肿瘤作用的研究[J]. .

Jun-Jun Xiao, Jing-Rong Cui, Ya-Xian Su, Jie He, Jun-En Yao

. Investigation on the Antitumor Activity of Organotin Compound[J]. .

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