关键词: 单动物个体—单观测值, 型数据, 有限重复抽样, 药物动力学参数, NONMEM

Abstract:

During pre-clinical pharmacokinetic research, it is not easy to gather complete pharmacokinetic data in each animal. In some cases, an animal can only provide a single observation. Under this circumstance, it is not clear how to utilize this data to estimate the pharmacokinetic parameters effectively. This study was aimed at comparing a new method to handle such single-observation-per-animal type data with the conventional method in estimating pharmacokinetic parameters. We assumed there were 15 animals within the study receiving a single dose by intravenous injection. Each animal provided one observation point. There were five time points in total, and each time point contained three measurements. The data were simulated with a one-compartment model with first-order elimination. The inter-individual variabilities (IIV) were set to 10%, 30% and 50% for both clearance (CL) and apparent volume of distribution (V). A proportional model was used to describe the residual error, which was also set to 10%, 30% and 50%. Two methods (conventional method and the finite resampling method) to handle with the simulated single-observation-per-animal type data in estimating pharmacokinetic parameters were compared. The conventional method (M1) estimated pharmacokinetic parameters directly with original data, i.e., single-observation-per-animal type data. The finite resampling method (M2) was to expand original data to a new dataset by resampling original data with all kinds of combinations by time. After resampling, each individual in the new dataset contained complete pharmacokinetic data, i.e., in this study, there were 243 () kinds of possible combinations and each of them was a virtual animal. The study was simulated 100 times by the NONMEM software. According to the results, parameter estimates of CL and V by M2 based on the simulated dataset were closer to their true values, though there was a small difference among different combinations of IIVs and the residual errors. In general, M2 was less advantageous over M1 when the residual error increased. It was also influenced by the levels of IIV as higher levels of IIV could lead to a decrease in the advantage of M2. However, M2 had no ability to estimate the IIV of parameters, nor did M1. The finite resampling method could provide more reliable results compared to the conventional method in estimating pharmacokinetic parameters with single-observation-per-animal type data. Compared to the inter-individual variability, the results of estimation were mainly influenced by the residual error.

Key words: Single-observation-per-animal type data, Finite resampling, Pharmacokinetic parameters, NONMEM