RP-HPLC-DAD法同时测定吴茱萸汤中7个生物碱和2个黄酮苷含量

doi:10.5246/jcps.2012.04.046

RP-HPLC-DAD法同时测定吴茱萸汤中7个生物碱和2个黄酮苷含量

胡传芹, 杨秀伟^*

北京大学医学部天然药物及仿生药物国家重点实验室; 药学院天然药物学系, 北京 100191

收稿日期:2012-02-29 修回日期:2012-05-08 出版日期:2012-06-25 发布日期:2012-06-25
通讯作者: 杨秀伟*

Simultaneous determination of seven alkaloids and two flavonoid glycosides in Wuzhuyu decoction by RP-HPLC-DAD

Chuan-Qin Hu, Xiu-Wei Yang^*

State Key Laboratory of Natural and Biomimetic Drugs; Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2012-02-29 Revised:2012-05-08 Online:2012-06-25 Published:2012-06-25
Contact: Xiu-Wei Yang*

摘要/Abstract

摘要：

建立反相高效液相色谱-二极管阵列检测 (RP-HPLC-DAD) 法同时测定经典名方吴茱萸汤中7个生物碱 (去氢吴茱萸碱、10-羟基吴茱萸次碱、吴茱萸碱、吴茱萸次碱、1-甲基-2-正壬基-4(1H)喹诺酮、吴茱萸卡品碱和二氢吴茱萸卡品碱) 和2个黄酮苷 (异鼠李素-7-O-芸香糖苷和香叶木素-7-O-β-D-吡喃葡萄糖苷)的含量。RP-HPLC分离应用反相C18色谱柱, 甲醇-1%醋酸水溶液梯度洗脱, 流速为1.0 mL/min, 检测波长为300 nm。上述9个分析物达到基线分离, 标准曲线线性关系良好(r²>0.9941), 日内和日间精密度和准确度符合分析方法学要求, 加样回收率为90.13%-102.48% (RSDs<3.6%)。经方法学验证, 所建立的方法适用于吴茱萸汤物质基础的质量控制。

关键词: 高效液相色谱, 吴茱萸汤, 生物碱, 黄酮苷

Abstract:

A reversed-phase high-performance liquid chromatography (RP-HPLC) method with diode array detector was established to simultaneously determine nine bioactive compounds in Wuzhuyu decoction, namely dehydroevodiamine, isorhamnetin-7-O-rutinoside, 10-hydroxyrutaecarpine, diosmetin-7-O-β-D-glucopyranoside, evodiamine, rutaecarpine, 1-methyl-2-n-nonyl-4(1H)quinolone, evocarpine, and dihydroevocarpine. The RP-HPLC assay was performed on a reversed-phase C18 column with a gradient elution. The mobile phases consisted of methanol and water containing 1% (v/v) acetic acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 300 nm. All calibration curves showed good linearity (r²>0.9941) within test ranges. The results of intra-day precision, inter-day precision, and accuracy were satisfactory. The overall recoveries were in the range of 90.13%-102.48%. The method was successfully applied to quantify nine bioactive compounds in six samples. Baseline resolution for all nine major compounds was achieved. This method could be used as a suitable quality control assay for Wuzhuyu decoction.

Key words: High-performance liquid chromatography, Wuzhuyu decoction, Alkaloids, Flavonoid glycosides

中图分类号:

R931

Supporting:

Foundation items: National Sciences and Technology Program of China (Grant No. 2011BAI07B08, 2006BAI06A01-02, 2006BAI08B03-09).
^*Corresponding author. Tel.: 86-10-62070317; Fax: 86-10-82802724

胡传芹, 杨秀伟^*. RP-HPLC-DAD法同时测定吴茱萸汤中7个生物碱和2个黄酮苷含量[J]. , DOI: 10.5246/jcps.2012.04.046.

Chuan-Qin Hu, Xiu-Wei Yang^* . Simultaneous determination of seven alkaloids and two flavonoid glycosides in Wuzhuyu decoction by RP-HPLC-DAD[J]. , DOI: 10.5246/jcps.2012.04.046.

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