Journal of Chinese Pharmaceutical Sciences

Contents list

2003, 12(4): 1-01.

Asbtract ( 652 )

PDF (278KB) ( 407 )

Related Articles | Metrics

Purification and Characterization of Hepatocyte Regeneration Stimulatory Factor from Shark Liver

OU Yu, LI Qian, LU Zheng-bing, WU Wu-tong^*, WANG Qiu-juan

2003, 12(4): 175-180.

Asbtract ( 1896 )

PDF (453KB) ( 526 )

References | Related Articles | Metrics

Aim To purify hepatocyte regeneration stimulatory factor from shark liver and research its molecular feature and activity. Methods and Results Hepatocyte regeneration stimulatory factor (sHRSF) was isolated from healthy shark livers and separated by homogenization, freezing-melting, heat treating, centrifugation, and ultrafiltration. HRSF activity was found mainly in the subfraction of molecular weight less than 30 000 daltons. This crude ultrafiltrate was further purified successively by DEAE-Sepharose fast flow chromatography, FPLC Resource 30Q, Resource Q and Mono Q chromatography. A single band was displayed on sodium dodecyl sulfate polyacrylamide gel electrophoresis, which corresponds to molecular weight of 14 600 daltons. The characteristic absorption was obtained at the wavelength 276 nm. The isoelectric point was about 5.1. It contained 18 amino acids and the 15 N-terminal amino acid residues were LVGPIGAVGPAGKDG. It had a significant activity in stimulating liver to regenerate. Conclusion We obtained an unknown new active protein, that is hep-atocyte regeneration stimulatory factor from shark liver (sHRSF).

A Monoterpene Glycoside from Ehinacea purpurea

LI Ji-ren, BAI Yan-jing, WANG Bin, CHEN Gang, AI Tie-min, ZHAO Yu-ying^*

2003, 12(4): 181-183.

Asbtract ( 799 )

PDF (423KB) ( 685 )

References | Related Articles | Metrics

Aim To separate and identify chemical constituents of Ehinacea purpurea. Methods Five compoundswere isolated from the plant using chromatography. Their structures were elucidated by spectroscopy. Results Five com-pounds were isolated and their structures were identified as 2, 6-dimethyl-7-octene-2, 3, 6-triol-2-O-β-D-glucopyranoside (1), 7, 8-furocoumarin (2), 6-methoxy-7-hydroxycoumarin (3), caffeic acid (4), methyl caffeate (5), and ethyl caffeate (6). Conclusion All these compounds were obtained from the plant for the first time.

Anticancer Activities of Substituted Cinnamic Acid Phenethyl Esters on Human Cancer Cell Lines

LI Shu-chun, LI Hui, ZHANG Fa, LI Zhong-jun^*, CUI Jing-rong^*^*

2003, 12(4): 184-187.

Asbtract ( 1534 )

PDF (369KB) ( 773 )

References | Related Articles | Metrics

Caffeic acid phenethyl ester (CAPE) and sixteen substituted cinnamic acid phenethyl esters were prepared via conventional procedures in order to test their in vitro anticancer activities by either MTT assay or SRB assay on six different human cancer cell lines. The results indicated that in the concentration of 10 μmol·L-1 the lead compound CAPE possessed anticancer activities against human HL-60, Bel-7402, and Hela cell lines, and two other compounds possessed potent anticancer activities against Bel-7402 and Hela cell lines.

Constructing Biophore of Uroselective α1-Adrenoceptor antagonist

FANG Hao, LU Jing-fen^*, XIA Lin

2003, 12(4): 188-191.

Asbtract ( 1006 )

PDF (417KB) ( 907 )

References | Related Articles | Metrics

Aim The biophore of uroselective α1-adrenoceptor antagonist was studied by using Apex-3D software on an O2 Silicon Graphics Computer Station. Methods Five known antagonists (Indoramin, GG-818, RS100975, R-YM12167, and KMD-3213), which possess both good selectivity and high affinities to prostate and α1-AR subtype, were chosen for building the biophore. Using an automatic filtering software for obtaining reasonable biophores, the filter parameters were se-lected: P (probability)>0.8, active (number of active compounds)≥4, and size (descriptor center)≥3. Results Three biophores conformed to the requirements, each of whom contained a basic center, an aromatic ring center and H-site according to the structure-activity relationships of known α1-adrenoceptor antagonist. Conclusion The biophore model de-veloped by computer simulation with Apex-3D software can be used to design and synthesize a new α1-adrenoceptor antago-nist with high activity and low side effect.

Determination of Five Organic Acids in Radix Isatidis by Column Partition Chromatography and Capillary Zone Electrophoresis

CHAI Yi-feng^*, JI Song-gang, ZHANG Guo-qing, LIU Chang-hai

2003, 12(4): 192-195.

Asbtract ( 904 )

PDF (369KB) ( 551 )

References | Related Articles | Metrics

Aim To determine five organic acids in Radix Isatidis. Method The extraction method and the column partition chromatographic conditions were studied. Then a capillary zone electrophoretic method was set up for the determina-tion. Results The linear ranges of quinazolinone acid, n-anthranilic acid, benzoic acid, salicylic acid, and syringic acid were 5.52-92.0 μg·mL^-1, 5.12-102 μg·mL^-1, 2.28-84.4 μg·mL^-1, 4.78-159 μg·mL^-1, and 1.74-87.0 μg·mL^-1 respectively. Conclusion The established method is accurate and simple.

Assignments of ¹H and ¹³C NMR Signals of Mogroside IVa

ZHANG Jian-ye, YANG Xiu-wei^*

2003, 12(4): 196-200.

Asbtract ( 1362 )

PDF (474KB) ( 665 )

References | Related Articles | Metrics

Aim To investigate the structure of mogroside Iva isolated from traditional Chinese medicine fructus mo-mordicae [fruits of Siraitia grosvenori (Swingle) C. Jeffery] and summarize the NMR characteristics of the structure. Methods Common extraction, separation and purification methods were used. Various NMR techniques including ¹H NMR,¹³C NMR, DEPT, ¹H-¹H COSY, HSQC, HMBC, NOESY and molecular model simulated by computer were used to elucidatethe structure. Results ¹H and ¹³C NMR signals of mogroside IVa were assigned, and spectroscopic basis was obtained for identification of such type of compounds. Conclusion 1D and 2D NMR techniques including ¹H-¹H COSY, HSQC, HM-BC, NOESY spectra are powerful tools for structure analysis. The structure determined by NMR methods is identical with energy minimized conformation simulated by computer.

A Sensitive Competitive ELISA for Determination of Biotin in Transformed Yeast Culture Media

YANG Hong^*

2003, 12(4): 201-206.

Asbtract ( 1416 )

PDF (391KB) ( 599 )

References | Related Articles | Metrics

Aim To develop a sensitive competitive ELISA for the determination of biotin in transformed yeast culture media. Methods The ELISA plate was firstly coated with Mycoplasma hyopneumoniae, and then successively incubated with rabbit anti-Mycoplasma hyopneumoniae serum and goat anti-rabbit IgG-biotin to form the solid biotin, which competed with the biotin in the solution (standard or sample) for the limited streptavidin-horse radish peroxidase conjugate. The stan-dard calibration curve for biotin analysis was constructed in the range of 50-2000 ng·L^-1. Results The detection limit for biotin was found to be 83 ng·L^-1, which was about 1000 times lower than the lowest determination concentration in the re-ported ELISA for biotin analysis. The relative standard deviations for the spiked samples at biotin concentrations of 200 ng·L^-1, 500 ng·L^-1, and 1000 ng·L^-1 were 24.87%, 6.15%, and 7.86%, respectively, with the average recovery of 101.13%. The wild yeast and its sixty-three transformed yeast culture media were applied to the developed ELISA for the determination of biotin. It was found that the biotin concentrations in more than 85 % of the tested samples were enhancedwith different increase factors after transformation. Conclusion Utilization of Mycoplasma hyopneumoniae as the coating protein improves the precision and accuracy of the ELISA assay, which might be used for the biotin assay in other media.

Swelling and Drug Release Characteristics of Poly (methacrylic acid-co-poloxamer) hydrogels

XU Hui^*, LIN Yan-nan, DING Ping-tian, TIAN Mei-juan, ZHENG Jun-min

2003, 12(4): 207-210.

Asbtract ( 982 )

PDF (459KB) ( 518 )

References | Related Articles | Metrics

Poly (methacrylic acid co-poloxamer) hydrogel networks were synthesized by free-radical solution polyme-rization, and the dynamic swelling and in vitro release properties of model drugs, dextromethorphan hydrobromide (DMP) and vitamin B12 (VB12) were studied. These gels exhibited pH-dependant swelling and sustained drug release properties, and the water uptake rate and drug release rate in neutral or basic media were higher than that in acidic media. The results showed that the water uptake followed non-Fickian or zero order process in neutral or basic media, and the release of model drugs from hydrogels of appropriate composition was of zero order kinetics over a period of several hours.

Development and Validation of a Novel Reverse Phase HPLC for Determination of Aloperine in Aloperine Tablets

WANG Hua, ZHENG Li, WANG Zhan, ZHANG Yong-qiang, WU Feng-lan^*

2003, 12(4): 211-214.

Asbtract ( 294 )

PDF (374KB) ( 704 )

References | Related Articles | Metrics

Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and antiinflamma-tory actions. Methods The samples were analyzed on a ODS column with methanol-water-triethylamine (3:97:0.1 V/V) as a mobile phase. The flow rate was 1.0 mL· min^-1, and UV detection wavelength 205 nm. Results Linear regression equation was A = 1.6920C + 1.7455 (r² = 0.9999, n = 5 ) in concentratins ranging from 20 to 120 μg·mL^-1. The recover-ies were 101.2+ 1.46 % at 80 μg·mL^-1, 100.5 +0.75% at 100 μg·mL^-1, and 100.7 + 1.10% at 120 μg·mL^-1, respectively, and the precisions of aloperine within or between run were from 0.80% to 1.98% ( n = 5). The relative cont-ents of aloperine in three lots of tablets were 101.59 + 1.38%, 98.46 + 0.23%, and 99.41 + 1.09% ( n = 3). Conclusion The newly developed reverse phase HPLC method is simple and useful for daily assay of aloperine tablets and can overcome the interference from excipient and other alkaloids in titration and UN detection.

Effect of 8-Chloroadenosine on Undifferentiatied HL-60 Cell Line

CUI Jing-rong, HUI Yu, XIANG You-qing, ZHANG Li-he^*

2003, 12(4): 215-221.

Asbtract ( 1370 )

PDF (748KB) ( 540 )

References | Related Articles | Metrics

Aim To study the effect of 8-chloroadenosine (8-CA)on undifferentiatied HL-60 cell line. Methods The IC50 of cancer cell proliferation was determined using a microculture plate reader at 570 nm (MTT) and 540 nm (SRB). Morphology of HL-60 cells was observed under a scanning electron microscope and a transmission electron microscope. The differentiation of HL-60 cells was examined by nitro blue tetrazolium reduction (NBT) and acid phosphatase assay. The cy-cle of HL-60 cells was analyzed by flow cytometry. Results 8-CA inhibited proliferation of eight human cancer cell lines.The IC50 ranked in the following order: KB (0.05μmol·L^-1)<HL-60(0.25 μmol·L^-1)<Bel-7402(0.56μmol·L^-1)<MCF(0.65μmol·L^-1)<HCT(0.79μmol·L^-1)<Hela(0.89μmol·L^-1)<BGC-823(1.49μmol·L^-1)<PG(2.50μmol·L^-1). The scanning and transmission electron microscopy showed that the microvilli of HL-60 cell surface shortened, and the shape of HL-60 cells nuclei changed to kidney-shaped, horse shoe-shaped and bilob ated after treatment with 8-CA. Meanwhile, 8-CA promoted NBT reduction and increased activity of acid phosphatase in HL-60 cells in a time and concentration-dependent manner. Flow cytometry analysis indicated that 8-CA induced an appreciable increase of the cell population in G1 phase with a marked reduction in S phase. Conclusion 8-CA can induce differentiation of HL-60 cells and block the cells at G1 phase, thus inhibiting proliferation of HL-60 cells.

Mechanism Underlying Protective Effect of Danbiqing Granule on Experimental Acute Bacterial Cholangitis in Rabbits

LIN Xiu-zhen^*, GONG Yan-ling, WANG Hong-bo

2003, 12(4): 222-226.

Asbtract ( 1007 )

PDF (440KB) ( 561 )

References | Related Articles | Metrics

Aim To study the mechanism of protective effects of Danbiqing granule (DBQ) on experimental acute bac-terial cholangitis in rabbits. Methods The acute bacterial cholangitis was induced by injecting 1 mL of 1 × 108 cfu·mL^-1 Escherchia coli suspension into common bile duit. The serum nitrous oxide (NO) levels were measured using nitric acid reductase kit. Phospholipase A2 (PLA2) activity was assayed by a method of acid titration (microassay). Serum tumor necrcsis factor-α (TNF-α), inferleukin-6 (IL-6) and plasma thromboxane B2 (TXB2), 6-keto-platelet growth factor 1 (PGF1α) were determined by radioimmunoassay. Results Compared with control group, serum NO, PLA2, TNF-α, IL-6and plasma TXB2 levels increased significantly in model group (P<0.01) while those of DBQ groups decreased significantly (P<0.01). Conclusion DBQ dramatically inhibits the overproduction of pro-inflammatory factor PLA2 and inflammatory cytokine. Hence, the mechanism of DBQ underlying anti-inflammatory and protective effect against acute bacterial cholangi-tis in rabbits has been revealed.

Antiviral Effects of Interferons and Their Therapeutic Potentials for SARS

YANG Li-ping, CAO Su-yan, SHAO Hong^*

2003, 12(4): 227-230.

Asbtract ( 834 )

PDF (360KB) ( 555 )

References | Related Articles | Metrics

Aurantiamide Acetate from Stems of Zanthoxylum Dissitum Hemsley

TANG Jun^*, Supinya Tewtrakul, WANG Zheng-tao, TU Zhi-ben

2003, 12(4): 231-233.

Asbtract ( 1233 )

PDF (355KB) ( 990 )

References | Related Articles | Metrics

CONTENTS OF VOLUME 12
AUTHOR INDEX

2003, 12(4): 234-238.

Asbtract ( 645 )

PDF (364KB) ( 709 )

Table of Content