Mechanism Underlying Protective Effect of Danbiqing Granule on Experimental Acute Bacterial Cholangitis in Rabbits

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Mechanism Underlying Protective Effect of Danbiqing Granule on Experimental Acute Bacterial Cholangitis in Rabbits

LIN Xiu-zhen^*, GONG Yan-ling, WANG Hong-bo

1.Department of Pharmacology, Tianjin Medical University, Tianjin 300070, China;
2.Jimo People's Hospital, Qingdao 266000, China

Received:2003-04-15 Revised:2003-11-10 Online:2003-12-15 Published:2003-12-15
Contact: LIN Xiu-zhen*

Abstract

Abstract: Aim To study the mechanism of protective effects of Danbiqing granule (DBQ) on experimental acute bac-terial cholangitis in rabbits. Methods The acute bacterial cholangitis was induced by injecting 1 mL of 1 × 108 cfu·mL^-1 Escherchia coli suspension into common bile duit. The serum nitrous oxide (NO) levels were measured using nitric acid reductase kit. Phospholipase A2 (PLA2) activity was assayed by a method of acid titration (microassay). Serum tumor necrcsis factor-α (TNF-α), inferleukin-6 (IL-6) and plasma thromboxane B2 (TXB2), 6-keto-platelet growth factor 1 (PGF1α) were determined by radioimmunoassay. Results Compared with control group, serum NO, PLA2, TNF-α, IL-6and plasma TXB2 levels increased significantly in model group (P<0.01) while those of DBQ groups decreased significantly (P<0.01). Conclusion DBQ dramatically inhibits the overproduction of pro-inflammatory factor PLA2 and inflammatory cytokine. Hence, the mechanism of DBQ underlying anti-inflammatory and protective effect against acute bacterial cholangi-tis in rabbits has been revealed.

Key words: cholangitis, cholangitis, Danbiqing granule, Danbiqing granule, nitrous oxide, nitrous oxide, phospholipase A2, phospholipase A2, thromboxane B2, thromboxane B2, tumor necrcsis factor-α, tumor necrcsis factor-α

CLC Number:

R285

R965

Supporting:

LIN Xiu-zhen^*, GONG Yan-ling, WANG Hong-bo . Mechanism Underlying Protective Effect of Danbiqing Granule on Experimental Acute Bacterial Cholangitis in Rabbits[J]. .

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