Determination of Five Organic Acids in Radix Isatidis by Column Partition Chromatography and Capillary Zone Electrophoresis

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Determination of Five Organic Acids in Radix Isatidis by Column Partition Chromatography and Capillary Zone Electrophoresis

CHAI Yi-feng^*, JI Song-gang, ZHANG Guo-qing, LIU Chang-hai

1.School of Pharmacy, Second Military Medical University, Shanghai 200433, China;
2.Department of Pharmacy, Eastern Institute and Hospital of Hepatobiliary Surgery, Shanghai 200438, China;
3.The 401st Hospital of PLA, Qingdao 266071, China

Received:2003-06-20 Revised:2003-11-10 Online:2003-12-15 Published:2003-12-15
Contact: CHAI Yi-feng*

Abstract

Abstract: Aim To determine five organic acids in Radix Isatidis. Method The extraction method and the column partition chromatographic conditions were studied. Then a capillary zone electrophoretic method was set up for the determina-tion. Results The linear ranges of quinazolinone acid, n-anthranilic acid, benzoic acid, salicylic acid, and syringic acid were 5.52-92.0 μg·mL^-1, 5.12-102 μg·mL^-1, 2.28-84.4 μg·mL^-1, 4.78-159 μg·mL^-1, and 1.74-87.0 μg·mL^-1 respectively. Conclusion The established method is accurate and simple.

Key words: Radix Isatidis, Radix Isatidis, organic acids, organic acids, capillary electrophoresis, capillary electrophoresis, column partition chromatography, column partition chromatography

CLC Number:

R917.1

Supporting:

CHAI Yi-feng^*, JI Song-gang, ZHANG Guo-qing, LIU Chang-hai . Determination of Five Organic Acids in Radix Isatidis by Column Partition Chromatography and Capillary Zone Electrophoresis[J]. .

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Determination of Five Organic Acids in Radix Isatidis by Column Partition Chromatography and Capillary Zone Electrophoresis

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