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Protective effects of icariin on human umbilical vein endothelial cell injured by angiotensin II

Qiu-Juan Wang*, Zhi-Wei Pan, Yu Wang, Juan Yang, Ying Jia, Ling-Yi Kong   

  1. 1. Department of Physiology, China Pharmaceutical University, Nanjing 210009, China;
    2. Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China
  • Received:2007-09-17 Revised:2008-01-10 Online:2008-03-15 Published:2008-03-15
  • Contact: Qiu-Juan Wang*

Abstract:

To investigate the effects of icariin (ICA) on angiotensin II(Ang II)-induced injury in human umbilical vein endothelial cells line (ECV-304). The ECV-304 cells were cultured in vitro. After 24 h incubating with icariin, the model of AngII-induced injury in ECV-304 was established. The cell viability (MTT method), Lactate dehydrogenase (LDH) release and Nitric oxide (NO) production in the medium, the capacity of scavenging superoxide anion radicals (O2·–) and hydroxyl radicals (·OH) were measured. The activities of superoxide dismutase (SOD), total nitric oxide synthase (T-NOS), inducible nitric oxide synthase (iNOS) and constitutive nitric oxide synthase (cNOS) in the cells were determined. Compared with the Ang II-treated group, ICA can significantly raise the viability of EC, increase the activities of SOD, T-NOS and cNOS, increase the production of NO, enhance the capacity of scavenging superoxide anion radicals (O2·–) and hydroxyl radicals(·OH), and lower LDH leakage and iNOS activity. The results suggest that ICA can protect endothelial cells (ECV-304) from Ang II-induced injury.

Key words: Icariin, Icariin, Angiotensin II, Angiotensin II, Human umbilical vein endothelial cells line, Human umbilical vein endothelial cells line, Nitric oxide, Nitric oxide

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