Development of a fingerprint of Melilotus suaveolens Ledeb by high-performance liquid chromatography

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Development of a fingerprint of Melilotus suaveolens Ledeb by high-performance liquid chromatography

Guo-Hua Zheng^#, Gui-Hong Wang^#, Mu-Qin Wu, Zhi-Jun Huang, Jun-Yan Tao^*, Xiao-Yu Zhang, Qiong-Guang Zhang, Fei Xiao, Chong-Ming Li

1. School of Pharmacy, Hubei College of Traditional Chinese Medicine, Wuhan 430065, China

2. Department of Affiliated Hospital, Hubei College of Traditional Chinese Medicine, Wuhan 430061, China

3. Hubei and Wuhan Engineering Technology center of Modernization Traditional Chinese Medicine, Wuhan Jianmin Pharmacy of TCM Co. Ltd, Wuhan 430054, China

4. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China

5. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China

Received:2008-08-15 Revised:2009-02-10 Online:2009-03-15 Published:2009-03-15
Contact: Jun-Yan Tao*

Abstract

Abstract: A simple and accurate high-performance liquid chromatography method (HPLC) was described to generate the metabolite fingerprints of Melilotus. suaveolens Ledeb of different harvesting times and different parts of the plant. A suitable chromatographic system was established using a linear gradient elution with acetonitrile and 0.1% phosphoric acid (H3PO4) as the mobile phase and a detection wavelength of 210 nm. HPLC analysis identified 12 common peaks and 8 common fingerprint peaks among the different parts of the herb. We found that the constituents varied at different harvesting times and in different parts of the plant. The HPLC fingerprint method could be used to evaluate the quality of M. suaveolens Ledeb and Melilotus genus herbs. The same strategy can also be applied for the development of fingerprints for the quality control of other herbal medicines.

Key words: HPLC fingerprint, HPLC fingerprint, Melilotus suaveolens Ledeb, Melilotus suaveolens Ledeb, Quality control, Quality control

CLC Number:

R284.1

Supporting:

Guo-Hua Zheng^#, Gui-Hong Wang^#, Mu-Qin Wu, Zhi-Jun Huang, Jun-Yan Tao^*, Xiao-Yu Zhang, Qiong-Guang Zhang, Fei Xiao, Chong-Ming Li

Development of a fingerprint of Melilotus suaveolens Ledeb by high-performance liquid chromatography

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