A small molecule LS-1-2F promotes the endocytosis of macromolecules into tumor cells

doi:10.5246/jcps.2019.08.052

Abstract

Abstract:

In the previous research, we found that anticancer agent LS-1-2F could cause the vacuolation of tumor cells. Herein we investigated the effect of compound LS-1-2F on the endocytosis of macromolecules, including fluorescence quantum dots, human serum albumin, single-stranded RNA, and monoclonal antibody, into tumor cells. We found that LS-1-2F could accelerate the endocytosis of these large molecules by laser confocal microscope and flow cytometry. The effect of LS-1-2F on the improvement of the internalization efficiency of Herceptin biosimilar was particularly significant. Promoting endocytosis will help increase the efficiency of liquid-phase drug uptake in drug-resistant cancer cells and could potentially facilitate the use of drugs in nanoparticle delivery vehicles.

Key words: Methuosis, Macropinosomes, Endocytosis of macromolecule, Herceptin biosimilar

CLC Number:

R962

Supporting:

Yanyan Yang, Dengbo Ji, Simin Yang, Siwang Yu, Zhongjun Li, Xiangbao Meng. A small molecule LS-1-2F promotes the endocytosis of macromolecules into tumor cells[J]. Journal of Chinese Pharmaceutical Sciences, 2019, 28(8): 547-555.

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