Proteomic changes in rat kidney injured by adenine and the regulation of anemoside B4

doi:10.5246/jcps.2019.01.002

Abstract

Abstract:

Adenine is commonly used to establish the animal models for chronic kidney injury and its renal interstitial fibrosis. As an endogenous substance, adenine-induced kidney damage has not yet been fully studied and elucidated, except for inflammatory reaction. Here we analyzed the proteomics of kidney of rats after adenine overloading using LS-MS/MS assay, and observed the role of anemoside B4 (B4). The results showed that adenine could down-regulate 285 proteins and up-regulate 164 proteins in rat kidney tissue compared with the normal group. Down-regulated proteins mainly affected related pathways, such as energy metabolism, while up-regulated proteins affected inflammatory response pathways and metabolic pathways. B4 could significantly reverse the down-regulation of about 40 proteins, which were involved in mitochondria, redox processes, extracellular exosomes, acetylation and other signaling pathways. Simultaneously, B4 could inhibit the up-regulation of five proteins caused by adenine, which were involved in cell cycle, oocyte meiosis, PI3K-Akt and other signaling pathways. Further experimental results of mRNA expression using real-time PCR assay supported the proteomic analysis. Therefore, we proposed that the damage of rat kidney caused by adenine was more complicated, not only with an inflammatory reaction, but also with extensive effects to various metabolic processes in the body. This work provided a valuable clue for comprehensive understanding of adenine-induced renal damage.

Key words: Adenine, Chronic kidney injury, Proteome, Anemoside B4, Rat

CLC Number:

R962

Supporting:

Luling He, Qin Gong, Xuan Yu, Mulan Wang, Shasha Wong, Fan Lei, Hongwei Gao, Yingying Luo, Yulin Feng, Shilin Yang, Jun Li, Lijun Du. Proteomic changes in rat kidney injured by adenine and the regulation of anemoside B4[J]. Journal of Chinese Pharmaceutical Sciences, 2019, 28(1): 10-20.

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