Simultaneous quantification of eighteen constituents in traditional Chinese medicine XiaoShuanTongLuo by UPLC-MS/MS

doi:10.5246/jcps.2014.04.031

Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (4): 225-232.DOI: 10.5246/jcps.2014.04.031

• Original articles • Previous Articles Next Articles

Simultaneous quantification of eighteen constituents in traditional Chinese medicine XiaoShuanTongLuo by UPLC-MS/MS

Yan Zhao, Min Liu, Zhao Yang, Shaohua Wang, Yang Li, Guanhua Du^*

1. Medical College, Qingdao University, Qingdao 266071, China
2. Qingdao Municipal Hospital, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266011, China
3. Qingdao Institute for Drug Control, Qingdao 266071, China
4. National Centre for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050, China

Received:2013-11-21 Revised:2013-12-24 Online:2014-04-28 Published:2014-01-07
Contact: *Corresponding author. Tel./Fax: 86-10-63165184; E-mail: dugh@imm.ac.cn
About author:*Corresponding author. Tel./Fax: 86-10-63165184; E-mail: dugh@imm.ac.cn
Supported by:
National Natural Science Foundation of China (Grant No. 30630073).

Abstract

Abstract:

XiaoShuanTongLuo (XSTL) is an important multi-herbal formula widely used as a traditional Chinese medicine (TCM). Inthis paper, an ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric method (UPLC-ESI-MS/MS) was developed for the simultaneous identification and quantification of18chemical constituents in the extract of XSTL.The analysis was performed in multiple-reaction monitoring (MRM) mode using positive and negative electrospray ionization (ESI). A BEH C₁₈(2.1 mm×100 mm, 1.7 μm) column with a gradient mobile phaseof methanol–0.1% aqueous formic acid solution at a flow rate of 0.2 mL/min was used for separation. The standard curves of most components showed good linearity over the concentration range of 0.001–0.5 μg/mL withr = 0.9896–0.9996. The relative standard deviations (RSD) of intra-day and inter-day precision were all lower than 15% at low, middle, and high concentration levels. The RSD of stability analysis in 24 h were also lower than 15%. The validated method was employed to quantify 18 active components simultaneously in four different commercial brands of XSTL products in various dosage forms. The developed method was rapid, accurate, sensitive, and reproducible for the quality control of XSTL.

Key words: Traditional Chinese medicine, XiaoShuanTongLuo, Ultra-performance liquid chromatography, Triple quadrupole tandem mass spectrometer, Quality control

CLC Number:

R932

Supporting:

Yan Zhao, Min Liu, Zhao Yang, Shaohua Wang, Yang Li, Guanhua Du. Simultaneous quantification of eighteen constituents in traditional Chinese medicine XiaoShuanTongLuo by UPLC-MS/MS [J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(4): 225-232.

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Simultaneous quantification of eighteen constituents in traditional Chinese medicine XiaoShuanTongLuo by UPLC-MS/MS

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