Application of UHPLC-MS/MS method in determining the content of multi-ingredient in hospital preparations

doi:10.5246/jcps.2014.04.032

Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (4): 233-240.DOI: 10.5246/jcps.2014.04.032

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Application of UHPLC-MS/MS method in determining the content of multi-ingredient in hospital preparations

Zhiyan Lin, Rongfu Yang, Yuenian Tang, Huaiping Tian, Jian Zhang^*

1. Department of Pharmacy, Xinhua Hospital Affiliated to School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China
2. Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China

Received:2014-03-12 Revised:2014-03-24 Online:2014-04-28 Published:2014-04-10
Contact: *Corresponding author. Tel.: 86-21-25077162; 86-21-25077150; E-mail: zj_xhpharm@126.com; zhiyanlin@163.com
About author:*Corresponding author. Tel.: 86-21-25077162; 86-21-25077150; E-mail: zj_xhpharm@126.com; zhiyanlin@163.com
Supported by:
Shanghai Science and Technology Commission Research Fund (Grant No. 11DZ1972500), Shanghai Health Bureau of Traditional Chinese Medicine Research Fund (Grant No. 2012G003A) and Shanghai Municipal Education Commission of Outstanding Young Teachers in Special Fund (Grant No. ZZjdyx13092).

Abstract

Abstract:

In the present study, we established an ultra-high pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to simultaneously determine 11 compounds in two hospital preparations(Shegan mixture and Gandi capsules), including ephedrine, caffeic acid, ferulic acid, rutin, scutellarin, belamcandin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin. The chromatographic separation was conducted on ZORBAX SB-C₁₈ (2.1 mm×50 mm, 1.8 μm). Eleven analytes and IS were detected using ESI and MRM combined with positive and negative scanning switch. Caffeic acid, ferulic acid, scutellarin and belamcandin were detected using negative ion mode detection, whereas ephedrine, rutin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin were detected using positive ion mode detection. The quantification limits of ephedrine, caffeic acid, scutellarin, belamcandin, baicalin, baicalein, irisflorentin and wogonin were 4.90×10^-3 ng/mL, 7.80 ng/mL, 6.8 ng/mL, 5.3×10^-2 ng/mL, 4.20×10^-3 ng/mL, 4.6×10^-2 ng/mL, 1.44×10^-4 ng/mL, 4.85 ng/mL, 0.23 ng/mL, 3.18×10^-4 ng/mL and 2.95×10^-4 ng/mL, respectively. The detection limits of these components were 2.90×10^-4 ng/mL, 0.77 ng/mL, 2.0 ng/mL, 0.016 ng/mL, 1.3×10^-3 ng/mL, 3.33×10^-4 ng/mL, 4.32×10^-5 ng/mL, 1.46 ng/mL, 0.07 ng/mL, 9.5×10^-5 ng/mL and 8.84×10^-5 ng/mL, respectively. All calibration curves showed good linearity (R²>0.99) within the test range. The RSD values of intra-day and inter-day precision were less than 5%, and the average recovery rates of the 11 components ranged from 80% to 120%. In conclusion, our newly developed method was simple, rapid, sensitive and accurate. It could be used to determineephedrine, caffeic acid, ferulic acid, rutin, scutellarin, belamcandin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin in Shegan mixture and Gandi capsules, which was helpful for the quality control of drugs.

Key words: Shegan mixture, Gandi capsules, Ultra-high pressure liquid chromatography-tandem mass spectrometry, Determination of multi-ingredient, Quality control of Chinese patent drug

CLC Number:

R917

Supporting:

Zhiyan Lin, Rongfu Yang, Yuenian Tang, Huaiping Tian, Jian Zhang. Application of UHPLC-MS/MS method in determining the content of multi-ingredient in hospital preparations[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(4): 233-240.

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Application of UHPLC-MS/MS method in determining the content of multi-ingredient in hospital preparations

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