Abstract:

To develop a simple and rapid purification method of rohitukine from the stem bark of Dysoxylum binectariferum. A L9(3⁴) orthogonal test was designed to optimize the extraction condition. Rohitukine in the plant extract was purified by using solvent-solvent partition and cation exchange resion (CER). Five different types of packing materials, including XAD-2 resin, polyamide, Sephadex LH-20, ODS and CER, were compared and CER showed the best capacity for rohitukine separation. The purification procedure was optimized as follows: the plant material powder was extracted with 70% ethanol (v/m = 60) by ultrasonic agitation for 60 min, then the 70% ethanol extract was dissolved in aqueous solution (pH 1, adjusted with 0.5 mol/L HCl) and extracted with equal volume of n-butanol. The aqueous layer was retained and the pH was adjusted to 10 with 25% aqueous ammonia and a solventsolvent partition was performed with equal volume of n-butanol. The obtained n-butanol extract was dissolved in aqueous solution (pH 1, adjusted with 0.5 mol/L HCl), and purified by a CER column eluting with H2O and 70% ethanol (pH 10, adjusted with 25% aqueous ammonia), successively. Rohitukine existed in 70% ethanol eluate, with a purity up to 53.3%. The method developed in this study provides a simple and rapid approach for the preparation of rohitukine from the stem bark of D. binectariferum.

Key words: Rohitukine, Rohitukine, Dysoxylum binectariferum, Dysoxylum binectariferum, Purification, Purification, Liquid-liquid extraction, Liquid-liquid extraction, Cation exchange resin, Cation exchange resin, ,