Determination of tenuifolin in Polygalae Radix from different regions by RF-HPCE

doi:10.5246/jcps.2011.04.042

Abstract

Abstract: A simple, low-cost and effective reverse flow-high performance capillary electrophoresis (RF-HPCE) method was developed for the separation and determination of tenuifolin in Polygalae Radix. Through optimization of separation conditions, a buffer of 50 mmol/Lborax containing methanol (65:35, v/v) was selected for the separation of analytes. Regression equation revealed good linear relationship between peak area and concentration of the analyte in the range of 0.0563-0.9000 mg/mL (r = 0.9988). The recoveries ranged from 99.50%-104.7%. The results indicated that this method is simple, rapid and accurate. It can be used for the quality control of Polygalae Radix.

Key words: RF-HPCE, Tenuifolin, Polygalae Radix

CLC Number:

R284.2

Supporting:

Zeng-Yan Hao, Jie Wang, Yi-Ming Zhang, An-Jia Chen^*. Determination of tenuifolin in Polygalae Radix from different regions by RF-HPCE[J]. , DOI: 10.5246/jcps.2011.04.042.

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Determination of tenuifolin in Polygalae Radix from different regions by RF-HPCE

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