Abstract: Aim To study the chemical constituents of the active fraction of Gui-Zhi decoction. Method The constituents were isolated and purified by column chromatography, and their structures were identified on the basis of spectral analysis. Result Six compounds, formononetin(1), liquiritigenin(2), isoliquiritigenin(3), 6-gingerol (4), (3S,5S)-1-(4-hydroxy-3-methoxyphenyl)-decane-3,5-diol (5), (3R,5S)-1-(4-hydroxy-3-methoxyphenyl) -decane -3,5-diol (6) were obtained. Conclusion All those compounds were isolated from the active fraction of Gui-Zhi decoction for the first time.

Key words: Gui-Zhi decoction, Gui-Zhi decoction, Flavonoids, Flavonoids, Phenol, Phenol