Rapid detection of mitochondria in extracellular vesicles using nanoflow cytometry

doi:10.5246/jcps.2026.03.021

Abstract

Abstract:

Mitochondria-rich extracellular vesicles (mitoEVs) are increasingly recognized as key modulators of cellular metabolism and tissue repair, yet their study has been hindered by the lack of sensitive and quantitative detection strategies. Here, we introduced a nanoflow cytometry (nano FCM)–based analytical platform enabling rigorous quantification and phenotypic characterization of mitoEVs. By integrating refined instrument calibration, systematic reagent validation, and a streamlined EV isolation procedure, we achieved reliable detection of mitochondria-associated signatures within defined vesicle populations. This workflow delivered a robust and scalable framework that enhanced mitoEV analysis and supported future applications in mechanistic research and therapeutic development.

Key words: Mitochondria, Extracellular vesicles, Nanoflow cytometry

Supporting:

Yufang Sun, Hualong Yu, Shuxiang Song. Rapid detection of mitochondria in extracellular vesicles using nanoflow cytometry[J]. Journal of Chinese Pharmaceutical Sciences, 2026, 35(3): 315-323.

Figures/Tables 4

References 32

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