Quality evaluation of Polyporus umbellatus based on HPLC specific chromatogram and fingerprint analysis

doi:10.5246/jcps.2020.12.079

Journal of Chinese Pharmaceutical Sciences ›› 2020, Vol. 29 ›› Issue (12): 896-907.DOI: 10.5246/jcps.2020.12.079

• Original articles • Previous Articles Next Articles

Quality evaluation of Polyporus umbellatus based on HPLC specific chromatogram and fingerprint analysis

Weijuan Jia¹, Shizhong Chen¹, Jianhua Sun², Zongli Bai³, Yazhuo Huang¹, Yuankuan Zhang¹, Hong Wang¹^,^*()

1 Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
2 Shaanxi Hanwang Lueyang Traditional Chinese Medicine Technology Co., Ltd., Lueyang 724300, China
3 Kangmei Pharmaceutical Co., Ltd., Puning 515300, China

Received:2020-08-09 Revised:2020-10-20 Accepted:2020-10-24 Online:2020-12-30 Published:2020-12-30
Contact: Hong Wang

About author:

Hong Wang is an associate professor of Department of Natural Medicine, School of Pharmacy, Peking University, Beijing. Her research interests include active components in medicinal plants and quality evaluation system of traditional Chinese medicine. She has undertaken many scientific research projects of Ministry of Education of the People's Republic of China, National Major Scientific and Technological Special Project for "Significant New Drugs Development" during the Twelfth Five-year Plan Period, National Administration of Traditional Chinese Medicine and Chinese Pharmacopoeia Commission. She also won the Second Prize of the State Scientific and Technological Progress Award and the third prize of Beijing Science and technology Progress Award. She made a systematic study on the resources and quality standards of many kinds of TCMs, including Rubiae Radix Et Rhizoma, Magnoliae Officinalis Cortex, Pyrrosiae Folium, Mume Flos, Polyporus and Apocyni veneti Folium. The results of her researches were presented in the Chinese Pharmacopoeia or in the industry standard and applied in practice. Professor Hong Wang has published more than 80 articles, wrote and edited 8 professional books. She is the editorial board member of Chinese Journal of Experimental Traditional Medical Formulae, the peer review expert of Beijing Natural Science Foundation, and the member of council of the Medicinal Resources Branch of the China Medical Association of Minorities.

Abstract

Abstract:

To evaluate the quality of Polyporus umbellatus, we established a simple, repeatable and reliable method based on high performance liquid chromatography (HPLC) for specific chromatogram and fingerprint analysis, which was applied to analyze samples of medicinal materials and decoction pieces collected from different regions. Finally, ten characteristic peaks were designated in the specific chromatograms and applied to the authenticate identification of P. umbellatus samples. Nine common peaks were designated in the fingerprints, and then the similarities between 32 batches of samples were calculated. Among them, eight compounds were identified by HPLC-APCI-IT-TOF-MSn, four of which were identified in specific chromatograms and four in fingerprints. In the present study, we, for the first time, combined HPLC specific chromatograms and fingerprints for the species identification and quality evaluation of P. umbellatus. Collectively, our findings provided a new method for establishing a comprehensive quality standard of P. umbellatus.

Key words: Polyporus umbellatus, HPLC, Specific chromatogram, Fingerprint, APCI-IT-TOF-MSn, Quality evaluation

Supporting:

Weijuan Jia, Shizhong Chen, Jianhua Sun, Zongli Bai, Yazhuo Huang, Yuankuan Zhang, Hong Wang. Quality evaluation of Polyporus umbellatus based on HPLC specific chromatogram and fingerprint analysis[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(12): 896-907.

Figures/Tables 10

Table 1. Sample information.

Table 2. Compounds identified in specific chromatograms of P. umbellatus by HPLC-APCI-IT-TOF-MSn.

Table 3. Compounds identified in fingerprints of P. umbellatus by HPLC-APCI-IT-TOF-MSn.

Table 4. RRT of common peaks in the 32 batches of P. umbellatus.

Table 5. RPA of common peaks in the 32 batches of P. umbellatus.

Table 6. Similarity values of 16 batches of medicinal materials.

Table 7. Similarity values of 16 batches of decoction pieces.

References 13

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Quality evaluation of Polyporus umbellatus based on HPLC specific chromatogram and fingerprint analysis

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