Effect of Huanglian Jiedu Decoction on vascular endothelium factor and expression of RhoA protein factor in young spontaneously hypertensive rats

doi:10.5246/jcps.2019.02.011

Abstract

Abstract:

To investigate the mechanism of effect of Huanglian Jiedu Decoction (HLJD) on vascular endothelium, we assessed the protective effect of HLJD on vascular endothelium in spontaneously hypertensive rats (SHR) and the expression of RhoA in thoracic aorta. A total of 40 SHR rats were randomly and evenly divided into model group (SHR group), positive group (captopril group), HLJD high-dose group, and HLJD low-dose group. Simultaneously, 10 Wistar Kyoto rats were used in the blank group.All groups were treated by gavage for 6 weeks. The changes of nitric oxide synthase (NOS), von Willebrand factor (vWF), endothelin (ET-1) and calmodulin (CAM) in rat serum were tested by enzyme linked immunosorbent assay (ELISA) method. The expression of RhoA at the protein and mRNA levels in thoracic aorta was determined by Western blotting (WB) and quantitative real-time PCR, respectively. Compare with the blank group after 6 weeks, the levels of ET-l and VWF in serum of the model group were significantly increased (P<0.01), and the levels of NOS and CAM were significantly decreased (P<0.01). Conversely, the levels of ET-1 and vWF in the positive and HLJD groups were significantly decreased (P<0.01 or P<0.05), and the levels of NOS and CAM were significantly increased (P<0.01 or P<0.05) compared with the model group. The expression of RhoA at the mRNA and protein levels was decreased obviously (P<0.05) in HLJD high-dose group. The results shown that HLJDincreased diastolic factor (CAM and NOS) in the vascular endothelial of rats, leading to reduced contraction factor (ET-1 and vWF). HLJD revealed the preventive function in the vascular endothelial dysfunction of the early stage hypertension through adjusting secretion of blood vessel endothelium (BVE) relaxing factor and improving vascular endothelial function.The mechanism might be associated with the inhibition of the activity of RhoA protein factor.

Key words: Huanglian Jiedu Decoction, Young spontaneously hypertensive rats, BE relaxing factor, RhoA

CLC Number:

R284

Supporting:

Nan Zhang, Yiwen Gao, Chanmei Lv, Xiaoli Yang, Guihua Yue. Effect of Huanglian Jiedu Decoction on vascular endothelium factor and expression of RhoA protein factor in young spontaneously hypertensive rats[J]. Journal of Chinese Pharmaceutical Sciences, 2019, 28(2): 114-120.

References

[1] Taddei, S.; Ghiadoni, L.; Virdis, A.; Versari, D.; Salvetti, A. Clinical significance of the assessment of endothelial function. Ital. Heart J. Suppl. 2004, 5, 357.

[2] Yue, G.H.; Zhuo, S.Y.; Xia, M.; Zhang, Z.; Gao, Y.W. Effect of Huanglian Jiedu Decoction on Thoracic Aorta Gene Expression in Spontaneous Hypertensive Rats. Res. Article. 2014, 11, 1-9.

[3] Ridley, A.J. Rho family proteins :coordinating cell responses. Trends Cell Biol. 2011, 11, 471-477.

[4] Rolfe, B.E.; Worth, N.F.; World, C.J.; Campbell, J.H.; Campbell, G.R. Rhoa and vascular disease. Atherosclerosis. 2005, 183, 1-16.

[5] Zhu, H.X.; Pan, L.M.; Li, H. Chin. Tradit. Pat. Med. 2010, 32, 1815-1818.

[6] Cheng, J.; Wan, L.H.; Fan, A.L.; Xiong, W.B. Study on the protective effect of Pearl Capsule on vascular endothelial cells in SHR. Pharmacol. Clin. Chin. Mater. Med. 2009, 25, 68.

[7] Taddei, S.; Ghiadoni, L.; Virdis, A.; Versari, D.; Salvetti, A. Clinical significance of the assessment of endothelial function. Ital. Heart J. Suppl. 2004, 5, 357.

[8] Ma, Q.; Liao, H.L. Advances in research of vascular remodeling in hypertension. Asia Pac. Tradit. Med. 2016, 12, 58-59.

[9] Yue, G.H.; Mo, L.; Luo, Y. Effects of Yiqi Huoxue Jiedu Recipe on inflammatory factors and vascular von Willebrand factor levels in spontaneously hypertensive rats. J. Emerg. Tradit. Chin. Med. 2010, 19, 1365-1367.

[10] Chu, Y.F.; Gao, P.J. Pathway and hypertension. Chin. J. Hypertension. 2007, 15, 714-716.

[11] Berridge, M.J. Smooth muscle cell calcium activation mechanisms. J. Physiol. 2008, 586, 047-5061.

[12] Lu, W.; Hu, K.; Yu, X.; Lan, X. Research progress of RhoA/ROCK pathway in pathogenesis of hypertension. China Med. Herald. 2015, 12, 28-32.

[13] Friel, A.M.; Sexton, D.J.; O’reilly, N.W. RhoA/Rho kinase: human umbilical artery m RNA expression in normal and preeclamptic pregnancies and functional role in isoprost ane-induced vasoconstriction. Reproduction. 2006, 132, 169-176.

[14] Yi, S.L.; Kant ores, C.; Belcast ro, R.; Cabacungan, J.; Tanswell, A.K. Jankov, R.P. 8-Is op rost ane-induced endothelin-1 production by infant rat pulmonary artery smooth muscle cells is mediated by Rho-kinase. Free Radic. Biol. Med. 2006, 41, 942-949.

[15] Watanabe, Y.; Faraci, F.M.; Heistad, D.D. Activation of Rho-associated kinase during augment edcont raction of the basilar artery to serotonin after subarachnoid hemorrhage. Am. J. Physiol. Heart Circ. Physiol. 2005, 288, 2653-2658.

[16] Zhang, N.; Gao, Y.W.; Yue, G.H.; Zhang, Z. Effect of Huanglian Jiedu Decoction on ultrastructure of vascular structure in young spontaneously hypertensive rats. Lishizhen Med. J. Med. 2013, 24, 2333-2335.

[17] Gao, Y.W.; Zhang, N.; Zhang, Z.; Yue, G.H. Effect of Huanglian Jiedu Decoction on the expression of endothelial nitric oxide synthase in aorta of young spontaneously hypertensive rats. Lishizhen Med. J. Med. 2012, 23, 2684-2685.

[18] Yue, G.H.; Zhuo, S.Y. Effects of Yiqi Huoxue Jiedu Recipe on endothelial function in young spontaneously hypertensive rats. Chin. J. Exp. Tradit. Med. Form. 2010, 16, 154-156.