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Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (11): 770-772.DOI: 10.5246/jcps.2015.11.098

• Short communication • Previous Articles    

Microbial specific carboxylation of (–)-huperzine A by Streptomyces griseus

Ridao Chen1,2,3, Dan Xie1, Jungui Dai1,2,3*   

  1. 1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China
    2. Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China
    3. Key Laboratory of Biosynthesis of Natural Products of National Health and Family Planning Commission, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China
  • Received:2015-09-08 Revised:2015-09-16 Online:2015-11-20 Published:2015-09-25
  • Contact: Tel.: 86-10-63165195, E-mail: jgdai@imm.ac.cn
  • Supported by:
    National Natural Science Foundation of China (Grant No. 81072541).

Abstract:

In a scale-up microbial transformation of (-)-huperzine A (1) by Streptomyces griseus CACC 200300, a specific carboxylated derivative (2)was yielded together with two known hydroxylated metabolites. The structure of 2 was characterized as 16-carboxyl huperzine A on the basis of IR, HRMS and NMR spectroscopic data analysis.

Key words: Specific carboxylation, (-)-Huperzine A, Streptomyces griseus

CLC Number: 

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