Mitochondria as the main source of vanadyl acetylacetonate-induced reactive oxygen species generation in renal epithelial cell lines

doi:10.5246/jcps.2013.01.010

Abstract

Abstract:

In this study, we aimed to clarify the source of the reactive oxygen species (ROS) generation induced by vanadium compounds. We used vanadyl acetylacetonate (VO(acac)₂), a highly effective agent in controlling hyperglycemia, to determine the source of ROS generation in two renal cell lines LLC-PK₁ and MDCK. Four commonly fluorescent dyes were used to assess VO(acac)₂-induced H₂O₂ and ·O₂^- production and their location. It demonstrated that VO(acac)₂can induce significant ROS generation in both LLC-PK₁ and MDCK cells, which were primarily derived from mitochondria. The results obtained in this study raised the possibility to reduce ROS level induced by vanadium compounds locally and thus avoid affecting its activity.

Key words: VO(acac)2, Mitochondria, Reactive oxygen species, LLC-PK1 cells, MDCK cells

CLC Number:

R915

Supporting:

Congcong Hou, Gang Wang, Xiaogai Yang^*. Mitochondria as the main source of vanadyl acetylacetonate-induced reactive oxygen species generation in renal epithelial cell lines[J]. , DOI: 10.5246/jcps.2013.01.010.

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