Gadolinium-promoted angiogenesis involves the activation of PKCα/β2 and MAPKs in human umbilical vein endothelial cells

doi:10.5246/jcps.2013.01.009

Abstract

Abstract: Gadolinium has been widely used as a contrast agent for magnetic resonance imaging in clinical practice. Recently, it was reported that gadolinium is involved in nephrogenic systemic fibrosis, although the exact mechanism by which gadolinium triggers nephrogenic systemic fibrosis remains unclear. In this study, we show that gadolinium chloride (GdCl₃) induced human umbilical vein endothelial cells (HUVECs) to migrate in Matrigel and tubulogenesis during wound healing. Chick chorioallantoic membrane assay confirmed that GdCl₃ stimulates angiogenesis. Under the optimal angiogenic concentration of GdCl₃ (10 μM), intracellular calcium concentration and reactive oxygen species generation were elevated. Moreover, western blotting results indicate that in cells treated with GdCl₃, Ca²⁺-dependent PKCα/β₂ was phosphorylated, and MAPKs pathways were also activated. Taken together, GdCl₃ has a potential effect on angiogenesis in HUVECs, and the possible mechanisms may involve oxidative stress and calcium-related signaling pathways.

Key words: Gadolinium chloride, Angiogenesis, Reactive oxygen species, Signaling pathways, Human umbilical vein endothelial cells

CLC Number:

R915

Supporting:

Xiao Wan, Baodi Gou^*, Kui Wang^*. Gadolinium-promoted angiogenesis involves the activation of PKCα/β2 and MAPKs in human umbilical vein endothelial cells [J]. , DOI: 10.5246/jcps.2013.01.009.

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