Simultaneous determination of 15 bioactive compounds in Fufang Zhenzhu Tiaozhi capsules by HPLC-DAD-ELSD

doi:10.5246/jcps.2013.01.006

Abstract

Abstract:

Fufang Zhenzhu Tiaozhi capsules (FTZc), which is consisted of eight traditional Chinese herbal medicines and contains multiple bioactive ingredients, is a patented and clinically approved herbal formulation for the treatment of dyslipidemia. A feasible HPLC-DAD-ELSD method was developed to simultaneously determine 15 bioactive compounds (salidroside, specneuzhenide, magnoflorine, rosmarinic acid, salvianolic acid B, columbamine, jatrorrhizine, epiberberine, coptisine, palmatine, berberine, 5,7-dimethoxycoumarin, ginsenoside Rg1, ginsenoside Rb1 and oleanic acid ) in FTZc for its quality control. The multiple wavelength detection mode of DAD was used. The chromatographic separation was performed on an Ultimate XB C18 column with gradient elution. The mobile phase A (acetonitrile) and B (0.25% glacial acetic acid and 0.13% triethylamine in water, v/v) were run at a flow rate of 0.8 mL/min. The developed method showed good precision and accuracy with overall intra- and inter-day variations of 0.7%-1.9% and 0.6%-3.0%, respectively. The recoveries measured at three concentration levels, varied from 95.5% to 103.8%. The validated method was successfully applied for the simultaneous determination of 15 bioactive compounds in three batches of FTZc. The results suggested that the developed method was convenient and reliable, particularly suitable for the routine quality control of FTZc.

Key words: HPLC-DAD-ELSD, Fufang Zhenzhu Tiaozhi capsule, Quality control

CLC Number:

R284.1

Supporting:

Hui Fan, Jiao Guo^*, Yuanyuan Chen. Simultaneous determination of 15 bioactive compounds in Fufang Zhenzhu Tiaozhi capsules by HPLC-DAD-ELSD[J]. , DOI: 10.5246/jcps.2013.01.006.

References

[1] Guo, J. China Patent. 200410051250. June, 2005.

[2] Guo, J.; Bei, W.J.; Hu, Y.M.; Tang, C.P.; He, W.; Liu, X.B.; Huang, L.H.; Cao, Y.; Hu, X.G.; Zhong, X.L; Cao, L. J. Ethnopharmacol. 2011, 135, 299-307.

[3] Tang, F.T.; Guo, J.; He, W.; Wang, L.J.; Luo, D.S.; Bei, W.J. Chin. J. Integr. Med. 2011. Doi: 10.1007/S11655-011-0794-Z

[4] Wei, Y.J.; Qi, L.W.; Li, P.; Luo, H.W.; Yi, L.; Sheng, L.H. J. Pharm. Biomed. Anal. 2007, 45, 775-784.

[5] Zhong, X.L.; Guo, J.; Wang, L.Y.; Luo, D.S.; Bei, W.J.; Chen, Y.Y.; Yan, K.Q.; Peng, J.H. Chromatographia. 2012, 75, 111-129.

[6] Xia, W.; Sun, C.; Zhao, Y.; Wu, L. Phytomedicine. 2011, 18, 516-520.

[7] Wang, J.; Hu, J.H. China. J. China Mater. Med. 2006, 31, 588-590.

[8] Yokozawa, T.; Ishida, A.; Cho, E.J.; Nakagawa, T. Phytomedicine. 2003, 10, 17-22.

[9] Kong, W.J.; Wei, J.; Abidi, P.; Lin, M.; Inaba, S.; Li, C.; Wang, Y.L.; Wang, Z.Z.; Si, S.Y.; Pan, H.N.; Wang, S.K.; Wu, J.D.; Wang, Y.; Li, Z.R.; Liu, J.W.; Jiang, J.D. Nat. Med. 2004, 10, 1344-1351.

[10] Kong, W.; Wei, J.; Abidi, P.; Lin, M.; Inaba, S.; Li, C.; Wang, Y.; Wang, Z.; Si, S.; Pan, H.; Wang, S.; Wu, J.; Wang, Y.; Li, Z.; Liu, J.; Jiang, J.D. Eur. J. Pharmacol. 2011, 668, 467-471.

[11] Hung, T.M.; Lee, J.P.; Min, B.S.; Choi, J.S.; Na, M.; Zhang, X.; Ngoc, T.M.; Lee, I.; Bae, K. Biol. Pharm. Bull. 2007, 30, 1157-1160.

[12] Zhang, X.M.; Qu, S.C.; Sui, D.Y.; Yu, X.F.; Lv, Z.Z. China J. Chin. Mater. Med. 2004, 29, 1085-1088.

[13] Fadel, O.; El Kirat, K.; Morandat, S. Biochim. Biophys. Acta. 2011, 1808, 2973-2980.

[14] Yang, T.L.; Lin, F.Y.; Chen, Y.H.; Chiu, J.J.; Shiao, M.S.; Tsai, C.S.; Lin, S.J.; Chen, Y.L. J. Sci. Food. Agric. 2011, 91, 134-141.

[15] Buus, N.H.; Hansson, N.C.; Rodriguez-Rodriguez, R.; Stankevicius, E.; Andersen, M.R.; Simonsen, U. Eur. J. Pharmacol. 2011, 670, 519-526.

[16] Yao, X.; He, W.; Li, Y.; Yong, L.I.; Ma, J.C.; Yan, X.H. J. China. Pharm. 2011, 22, 1398-1400.

[17] Chen, Y.Y.; Guo, J.; Fan, H.; Huan, L.H. Chin. Herb. Med. 2012, 4, 230-236

[18] Wang, H.L.; Yao, W.F.; Zhu, D.N.; He, Y.Z. Chin. J. Nat. Med. 2011, 8, 343-348.

[19] Ye, J.; Zhang, X.; Dai, W.; Yan, S.; Huang, H.; Liang, X.; Li, Y.; Zhang, W. J. Pharm. Biomed. Anal. 2009, 49, 638-645.