Abstract: The aim of current study was to investigate the chemical components of the aerial part of Reineckia carnea, collected in Yunnan Province of China. Repeated column chromatography (CC) separations were performed to isolate and purify components. Compounds were identified by the analysis of their 1D and 2D NMR data as well as IR and MS spectra. A new pregnane-type glycoside, named 1α,3β-diol-5β-pregn-16-ene-20-one-1-O-α-L-arabinosyl-(1→2)-α-L-rhamnoside (1), together with β-amyrin (2), stigmasterol (3), α-spinasterol-3-O-β-D-glucoside (4), naringenin (5), β-sitosterol (6) and daucosterol (7), were isolated from ethyl acetate (EtOAc) and normal butanol (n-BuOH) extracts. Compounds 2, 4, 5 were isolated from this plant for the first time.

Key words: Reineckia carnea, Steroid saponin, Saponin