Fast and simultaneous determination of ginsenosides by isocratic high-performance liquid chromatography

Abstract

Abstract: A high-performance liquid chromatography method for the simultaneous determination of seven bioactive ginsenosides with diol stationary phase and isocratic elution was used to determin the ginsenosides in ginseng products. The optimization of the chromatographic separation was performed and the effect of temperature on separation was investigated. Using the validated procedure, the developed method was demonstrated to be more sensitive and effective than the conventional reversed-phase chromatography, where the chromatographic run is time-consuming to analyze a large number of ginsenosides. The results indicated that the developed method can be used for the quantitative determination of ginsenosides in complex ginseng samples.

Key words: Liquid chromatography, Pharmaceutical analysis, Quantification, Ginsenosides, Ginseng

CLC Number:

R284.1

Supporting:

Da-Wei Lou^* . Fast and simultaneous determination of ginsenosides by isocratic high-performance liquid chromatography[J]. .

References

[1] Yun, T.K. J. Korean Med. Sci. 2001, 16, S3-S5.
[2] Hu, S.Y. Econ. Bot. 1976, 30, 11-28.
[3] Hou, J.P. Am. J. Chin. Med. 1977, 5, 123-145.
[4] Liu, C.X.; Xiao, P.G. J. Ethnopharmacol. 1992, 36, 27-38.
[5] Sticher, O. Chemtech. 1998, 28, 26-32.
[6] Vongsangnak, W.; Gua, J.; Chauvatcharin, S.; Zhong, J. Biochem. Eng. J. 2004, 18, 115-120.
[7] Fuzzati, N.; Gabetta, B.; Jayakar, K.; Pace, R.; Peterlongo, F. J. Chromatogr. A. 1999, 854, 69-79.
[8] Chan, T.W.D.; But, P.P.H.; Cheng, S.W.; Kwok, I.M.Y.; Lau, F.W.; Xu, H.X. Anal. Chem. 2000, 72, 1281-1287.
[9] Corthout, J.; Naessens, T.; Apers, S.; Vlietinck, A.J. J. Pharm. Biomed. Anal. 1999, 21, 187-192.
[10] Vanhaelen-Fastre, R.; Faes, M.; Vanhaelen, M. J. Chromatogr. A. 2000, 868, 269-276.
[11] Bombardelli, E.; Bonati, A.; Gabetta, B.; Martinelli, E.M. J. Chromatogr. 1980, 196, 121-132.
[12] Cui, J.F.; Bjöerkhem, I.; Eneroth, P. J. Chromatogr. B. 1997, 689, 349-355.
[13] Lee, H.K.; Koh, H.L.; Ong, E.S.; Woo, S.O. J. Sep. Sci. 2002, 25, 160-166.
[14] Lau, A.J.; Woo, S.O.; Koh, H.L. J. Chromatogr. A. 2003, 1011, 77-87.
[15] Li, L.; Zhang, J.L.; Sheng, Y.X.; Ye, G.; Guo, D.A.; Wang, Q.; Guo, H.Z. J. Pharm. Biomed. Anal. 2005, 38, 45-51.
[16] Shangguan, D.; Han, H.; Zhao, R.; Zhao, Y.; Xiong, S.; Liu, G. J. Chromatogr. A. 2001, 910, 367-372.
[17] Kite, G.C.; Howes, M.J.R.; Leon, C.J.; Simmonds, M.S.J. Rapid Commun. Mass Spectrom. 2003, 17, 238-244.
[18] Wang, X.; Sakuma, T.; Asafu-Adjaye, E.B.; Shiu, G.K. Anal. Chem. 1999, 71, 1579-1584.
[19] Li, W.; Gu, C.; Zhang, H.; Awang, D.V.C.; Fitzloff, J.F.; Fong, H.H.S.; van Breemen, R.B. Anal. Chem. 2000, 72, 5417-5422.
[20] Ji, Q.C.; Harkey, M.R.; Henderson, G.L.; Gershwin, M.E.; Stern, J.S.; Hackman, R.M. Phytochem. Anal. 2001, 12, 320-326.
[21] Zhang, H.Z.; Wu, Y.J.; Cheng, Y.Y.; J. Pharm. Biomed. Anal. 2003, 31, 175-183.
[22] Ji, H.Y.; Lee, H.W.; Kim, H.K.; Kim, H.H.; Chang, S.G.; Sohn, D.H.; Kim, J.; Lee, H.S. J. Pharm. Biomed. Anal. 2004, 35, 207-212.
[23] Soldati, F.; Sticher, O. Planta Med. 1980, 38, 348-357.
[24] Pietta, P.; Mauri, P.; Rava, A. J. Chromatogr. 1986, 356, 212-219.
[25] Yamaguchi, H.; Kasai, R.; Matsuura, H.; Tanaka, O.; Fuwa, T. Chem. Pharm. Bull. 1988, 36, 3468-3473.
[26] Guldager Petersen, T.; Palmqvist, B. J. Chromatogr. 1990, 504, 139-149.
[27] Kanazawa, H.; Nagata, Y.; Kurosaki, E.; Matsushima, Y.; Takai, N. J. Chromatogr. 1993, 632, 79-85.
[28] Lou, D.W.; Saito, Y.; Jinno, K. Chromatographia. 2005, 62, 349-354.
[29] Li, L.; Zhang, J.L.; Sheng, Y.X.; Ye, G.; Guo, H.Z.; Guo, D.A. J. Chromatogr. B. 2004, 808, 177-183.
[30] Ligor, T.; Ludwiczuk, A.; Wolski, T.; Buszewski, B. Anal. Bioanal. Chem. 2004, 383, 1098-1105.
[31] Li, Y.G.; Liu, H.; Vander Heyden, Y.; Chen, M.; Wang, Z.T.; Hu, Z.B. Anal. Chim. Acta. 2005, 536, 39-48.
[32] Lou, D.W.; Saito, Y.; Zarzycki, P.K; Ogawa, M.; Jinno, K. Anal. Bioanal. Chem. 2006, 385, 96-104.
[33] Lou, D.W.; Saito, Y.; Jinno, K. Chromatographia. 2006, 63, 31-37.
[34] Fuzzati, N. J. Chromatogr. B. 2004, 812, 119-133.