Facile extraction of high-purity mangiferin from mango industrial wastes

doi:10.5246/jcps.2021.10.071

Abstract

Abstract:

In the present study, we described a novel, simple, and scalable method for isolating and purifying natural mangiferin from fresh mango leaves. An ultrasonic-assisted method was used to extract mangiferin from mango leaves using methanol as the solvent. The yields of the mangiferin crude extract and purified mangiferin recrystallized from a mixture of methanol and trichloromethane (10:1, v/v) were 1.41% (purity 86.5%) and 0.75% (purity > 99.6%), respectively. The purity of mangiferin was assessed by UV spectroscopy and HPLC. The structure of the purified compound was confirmed spectroscopically by UV, IR, Q-TOF/MS,¹H NMR, and¹³C NMR. The method described herein to isolate mangiferin was simple, inexpensive, rapid, and efficient. Moreover, we, for the first time, obtained mangiferin with a purity of more than 99.6% using this method.

Key words: Mango leaves, Mangiferin, Extraction, Recrystallization, Purity analysis

Supporting:

Chaohai Pang, Hai Tian, Xiaojie Guo, Pan Wang, Bingjun Han, Xinfeng Yang. Facile extraction of high-purity mangiferin from mango industrial wastes[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(10): 831-837.

Figures/Tables 5

References 18

[1]	Gómez-Caravaca, A.M.; López-Cobo, A.; Verardo, V.; Segura-Carretero, A.; Fernández-Gutiérrez, A. HPLC-DAD-q-TOF-MS as a powerful platform for the determination of phenolic and other polar compounds in the edible part of mango and its by-products (peel, seed, and seed husk). Electrophoresis. 2016, 37, 1072–1084.
[2]	He, L.Y.; Peng, X.F.; Zhu, J.F.; Chen, X.; Liu, H.; Tang, C.Y.; Dong, Z.; Liu, F.Y.; Peng, Y.M. Mangiferin attenuate sepsis-induced acute kidney injury via antioxidant and anti-inflammatory effects. Am. J. Nephrol. 2014, 40, 441–450.
[3]	Souza, J.R.R.; Trevisan, M.T.S.; Feitosa, J.P.A.; Ricardo, N.M.P.S.; Hull, W.E.; Erben, G.; Würtele, G.; Breuer, A.; Frei, E.; Ulrich, C.M.; Owen, R.W. Transformation of mangiferin to norathyriol by human fecal matrix in anaerobic conditions: comprehensive NMR of the xanthone metabolites, antioxidant capacity, and comparative cytotoxicity against cancer cell lines. Nat. Prod. Commun. 2020, 15. https://doi.org/10.1177/1934578X20910286.
[4]	Zhang, B.P.; Fang, J.; Chen, Y. Antioxidant effect of mangiferin and its potential to be a cancer chemoprevention agent. Lett. Drug Des. Discov. 2013, 10, 239–244.
[5]	Khurana, R.K.; Kaur, R.; Lohan, S.; Singh, K.K.; Singh, B. Mangiferin: a promising anticancer bioactive. Pharm. Pat. Anal. 2016, 5, 169–181.
[6]	Chavan, J.J.; Ghadage, D.M.; Kshirsagar, P.R.; Kudale, S.S. Optimization of extraction techniques and RP-HPLC analysis of antidiabetic and anticancer drug mangiferin from roots of ‘saptarangi’ (salacia chinensis L.). J. Liq. Chromatogr. Relat. Technol. 2015, 38, 963–969.
[7]	Kavitha, M.; Nataraj, J.; Essa, M.M.; Memon, M.A.; Manivasagam, T. Mangiferin attenuates MPTP induced dopaminergic neurodegeneration and improves motor impairment, redox balance and Bcl-2/Bax expression in experimental Parkinson’s disease mice. Chem. Biol. Interact. 2013, 206, 239–247.
[8]	Huang, C.P.; Luo, Q.; Li, J. Optimizing mangiferin extraction from Mangifera indica L. peel and analyzing its antibacterial activity. J. South. Agric. 2014, 45, 1452–1456.
[9]	Sai-Ut, S.; Benjakul, S.; Kraithong, S.; Rawdkuen, S. Optimization of antioxidants and tyrosinase inhibitory activity in mango peels using response surface methodology. LWT Food Sci. Technol. 2015, 64, 742–749.
[10]	Samadarsi, R.; Mishra, D.; Dutta, D. Mangiferin nanoparticles fortified dairy beverage as a low glycemic food product: its quality attributes and antioxidant properties. Int. J. Food Sci. Technol. 2020, 55, 589–600.
[11]	Zou, T.B.; Wu, H.F.; Li, H.W.; Jia, Q.; Song, G. Comparison of microwave-assisted and conventional extraction of mangiferin from mango (Mangifera indica L.) leaves. J. Sep. Sci. 2013, 36, 3457–3462.
[12]	Salomon, S.; Sevilla, I.; Betancourt, R.; Romero, A.; NuevasPaz, L.; AcostaEsquijarosa, J. Extraction of mangiferin from Mangifera indica L. leaves using microwave-assisted technique. Emir. J. Food Agric. 2014, 26, 616.
[13]	Wei, H.P.; Zheng, Y.; Han, H.B.; Shang, Y.H. Efficient extraction and isolation of mangiferin from mango leaves by ethyl acetate impurity removal method. Asian Agric. Res. 2018, 10, 56–60, 71.
[14]	Vo, T.H.T.; Nguyen, T.D.; Nguyen, Q.H.; Ushakova, N.A. Extraction of mangiferin from the leaves of the mango tree mangifera indica and evaluation of its biological activity in terms of blockade of α-glucosidase. Pharm. Chem. J. 2017, 51, 806–810.
[15]	Kulkarni, V.M.; Rathod, V.K. Extraction of mangiferin from Mangifera indica leaves using three phase partitioning coupled with ultrasound. Ind. Crops Prod. 2014, 52, 292–297.
[16]	Zhang, Q.; Wang, Z.; Song, L.; Le, L. Optimization of ultrasound assisted extraction of mangiferin from mango leaves with response surface methodology. Northwest. Pharm. J. 2014, 29, 237–241.
[17]	Yuan, Y.; Hu, X. Extraction and Separation of Mangiferin from Swertia chuanxiensis. Lishizhen Med. Mater. Med. Res. 2009, 20, 1.
[18]	Shahat, A.A.; Hassan, R.A.; Nazif, N.M.; Van Miert, S.; Pieters, L.; Hammuda, F.M.; Vlietinck, A.J. Isolation of mangiferin from Bombax malabaricum and structure revision of shamimin. Planta Med. 2003, 69, 1068–1070.