Lanthanum chloride promotes proliferation of NIH 3T3 cells via cell cycle machinery

doi:10.5246/jcps.2013.01.008

Abstract

Abstract:

Lanthanum ions (La³⁺) have been reported to exert profound effects on the proliferation of NIH 3T3 mouse embryonic fibroblasts. In the present studies, the pro-proliferative effect of La³⁺ on NIH 3T3 cells was further characterized, and its impacts on the cell cycle kinetics were analyzed in detail by cell cycle arrest-and-release and pulse BrdU-labeling and chasing experiments. The results show that La³⁺ promoted the proliferation of NIH 3T3 cells after 12 h and the EC50 was 2.4 μM after 48 h. Such effect was also confirmed by enhanced BrdU incorporation. La³⁺ stimulated more cells to pass through the G1/S checkpoint into S phase, but did not change the length of cell cycle. Furthermore, La³⁺-treatment increased the protein levels and nuclear localization of cyclin D1 and c-Myc, two key regulators of G1/S checkpoint, as demonstrated by immunostaining. The pro-proliferative effects of La³⁺ share striking similarity with that of Gd³⁺, and provided new information regarding the cellular biological activities of lanthanides.

Key words: Lanthanum ions, NIH 3T3 cells, Proliferation, Cell cycle, Cyclin D1, c-Myc

CLC Number:

Supporting:

Xin Pan, Jinxia Li, Faqin Dong, Jianjun Deng, Lan Yuan, Kui Wang, Siwang Yu^*. Lanthanum chloride promotes proliferation of NIH 3T3 cells via cell cycle machinery[J]. , DOI: 10.5246/jcps.2013.01.008.

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Lanthanum chloride promotes proliferation of NIH 3T3 cells via cell cycle machinery

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