羟乙酸磺酸戊烷脒(PI)对小鼠受精卵的影响

羟乙酸磺酸戊烷脒(PI)对小鼠受精卵的影响

王英, 张树成, 戴景峰, 邱劲松, 邱毅, 孙亦彬

国家计生委科研所, 北京 100081

收稿日期:1993-03-03 修回日期:1993-07-16 出版日期:1994-06-15 发布日期:1994-06-15

Effect of Pentamidine Isethionate (PI) on Fertilized Ova of ICR Mice

Ying Wang, Shu-Cheng Zhang, Jing-Feng Dai, Jin-Song Qiu, Yi Qiu, Yi-Bin Sun

National Research Institute for Family Planning, Beijing 100081

Received:1993-03-03 Revised:1993-07-16 Online:1994-06-15 Published:1994-06-15

摘要/Abstract

摘要： 一组ICR小鼠体内受精后取出受精卵; 另一组超排卵后取出卵, 与ICR雄鼠精子体外受精, 获得受精卵。将两种途径得到的受精卵分别与不同浓度的PI(0, 0.25, 0.5, 2.5, 5.0 μg/ml)作用15分钟后, 去除药液。受精卵继续培养。在28小时后观察其分裂情况。实验结果表明两种途径得到的受精卵在PI作用下正常卵裂率减少, 异常卵裂率增加, 与对照组相比均具有显著性差异(P<0.01), 并且随作用时间的延长异常程度加重。提示PI具有抗生育作用。

关键词: 羟乙酸磺酸戊烷脒, 受精卵, 分裂

Abstract: Two kinds of fertilized ova were collected .in vivo and in vitro fertilized ova. All the ova were incubated with 0, 0.25, 0.5, 2.5 and 5.0 μg/ml Pentamidine isethionate (PI) for 15 min, respectively. After drug treatment. the fertilized ova were further cultured for 28 h to investigatethe cleavage of ova. The results showed that the cleavage of fertilized ova in the PI-treated groupswere significantly reduced in normal cleavage and increased in abnormal cleavage as compared tothose in the controls (p<0.01). It may be concluded that PI possesses an antifertility effect.

Key words: Pentamidine isethionate, Fertilized ova, Cleavage

Supporting:

王英, 张树成, 戴景峰, 邱劲松, 邱毅, 孙亦彬. 羟乙酸磺酸戊烷脒(PI)对小鼠受精卵的影响[J]. .

Ying Wang, Shu-Cheng Zhang, Jing-Feng Dai, Jin-Song Qiu, Yi Qiu, Yi-Bin Sun. Effect of Pentamidine Isethionate (PI) on Fertilized Ova of ICR Mice[J]. .

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