肿瘤热疗用磁流体的制备和表征

肿瘤热疗用磁流体的制备和表征

周彦菲, 范田园^*, 杨仁杰, 孙宏亮, 夏启胜

1.北京大学药学院药剂学系, 北京 100083;
2.北京大学肿瘤医院介入治疗科, 北京 100036;
3.中日友好医院临床医学中心, 北京 100029

收稿日期:2007-03-20 修回日期:2007-08-10 出版日期:2007-09-15 发布日期:2007-09-15
通讯作者: 范田园*

Preparation and characterization of ferromagnetic fluids for hyperthermia of tumor

Yan-Fei Zhou, Tian-Yuan Fan^*, Ren-Jie Yang, Hong-Liang Sun, Qi-Sheng Xia

1. Department of Pharmacy, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
2. Department of Interventional Therapy, School of Oncology, Peking University, Beijing 100036, China;
3. Clinical Medical Science Institute, China Japan Friendship Hospital, Beijing 100029, China

Received:2007-03-20 Revised:2007-08-10 Online:2007-09-15 Published:2007-09-15
Contact: Tian-Yuan Fan*

摘要/Abstract

摘要： 目的制备和表征肿瘤热疗用磁流体。方法在聚乙二醇6000（PEG-6000）的存在下用化学共沉淀法制备肿瘤热疗用四氧化三铁(Fe3O4)磁流体, 用邻二氮菲显色法测定磁流体中铁的含量, 通过电子显微镜、X衍射、红外和振动样品磁强计对制备的磁流体进行表征。测定了磁流体在交变磁场作用下的热效应, 并将该磁流体用于VX2兔肿瘤的热疗。结果红外图谱和X衍射图谱证明所制备的磁流体样品为Fe3O4; 电镜照片显示磁性粒子近乎圆形且分散良好; 经X衍射数据计算得磁性粒子的粒径为13.3 ± 3.8 nm; 样品的饱和磁化强度和剩余磁化强度分别为23.39 A/m (1.556 emu/g) 和 0.56 A/m (0.02604 emu/g), 矫顽力为12Oe; 磁流体的特征吸收率为69 ± 10 W/g [Fe]。将该磁流体直接注射于VX2兔肝肿瘤部位后, 置于交变磁场中进行热疗, 测得肿瘤部位温度可达到41~46 °C。结论在PEG-6000存在下所制备的Fe3O4磁流体有望用于肿瘤热疗。

关键词: 铁磁流体, 铁磁流体, 铁磁流体, 四氧化三铁Fe3O4, 四氧化三铁Fe3O4, 四氧化三铁Fe3O4, 聚乙二醇PEG, 聚乙二醇PEG, 聚乙二醇PEG, 热效应, 热效应, 热效应, 热疗, 热疗, 热疗

Abstract: Aim To prepare and characterize ferromagnetic fluids for hyperthermia of tumor. Methods Ferromagnetic fluids (FFs) of magnetite (Fe3O4) was prepared in the presence of polyethylene glycol (PEG-6000) by chemical precipitation method. The iron content of the FFs was determined by spectrophotometric method using o-phenanthroline. The FFs/PEG-6000 was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), infrared spectrometry (IR), and vibrating sample magnetometer (VSM). Heating effects of the FFs was measured in an alternating magnetic field in vitro. The hyperthermia of FFs in a rabbit was performed. Results The FFs/PEG-6000 was proved to be composed of Fe3O4 by XRD and IR. TEM showed that the ferromagnetic particles appeared to be almost spherical and dispersed well. The average particle size was 13.3 ± 3.8 nm by XRD. The saturation magnetization and residual magnetization of the FFs were 23.39 A/m (1.556 emu/g) and 0.56 A/m (0.02604 emu/g), respectively. The coercive force was 12 Oe. The specific absorption rate (SAR) of FFs was 69 ± 10W/g [Fe]. After direct injection of FFs to hepatic VX2 carcinoma of a rabbit, the temperature in the core of the tumor was between 41 – 46 °C in an alternating magnetic field. Conclusion FFs/PEG-6000 was expected to be useful in hyperthermia of tumor.

Key words: Ferromagnetic fluids, Ferromagnetic fluids, Fe3O4, Fe3O4, Polyethylene glycol PEG, Polyethylene glycol PEG, Heating effects, Heating effects, Hyperthermia, Hyperthermia

中图分类号:

R943

Supporting: ^*Corresponding author. Tel./fax: 86-10-82801584

周彦菲, 范田园^*, 杨仁杰, 孙宏亮, 夏启胜. 肿瘤热疗用磁流体的制备和表征[J]. .

Yan-Fei Zhou, Tian-Yuan Fan^*, Ren-Jie Yang, Hong-Liang Sun, Qi-Sheng Xia. Preparation and characterization of ferromagnetic fluids for hyperthermia of tumor[J]. .

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