脂质包膜微泡超声造影剂基础物理和声学性质以及体外造影增强实验

脂质包膜微泡超声造影剂基础物理和声学性质以及体外造影增强实验

向宇, 孙剑, 齐宪荣^*

1. 北京大学药学院药剂学系, 北京 100083;
2. 中国科学院声学研究所, 北京 100080

收稿日期:2007-03-11 修回日期:2007-08-10 出版日期:2007-09-15 发布日期:2007-09-15
通讯作者: 齐宪荣*

Basic physical, acoustic properties, and in vitro ultrasound imaging enhancement of the lipid-coated microbubbles

Yu Xiang, Jian Sun, Xian-Rong Qi^*

1. Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
2. Institute of Acoustic, Chinese Academy of Sciences, Beijing 100080, China

Received:2007-03-11 Revised:2007-08-10 Online:2007-09-15 Published:2007-09-15
Contact: Xian-Rong Qi*

摘要/Abstract

摘要： 目的为了研究一种具有良好声学性质和增强造影效果的稳定微泡, 并使其能够运用在心血管系统疾病的诊断中, 增强超声显影效果, 设计本实验。方法对这种磷脂为膜材的微泡造影剂（Lipid coated microbubble, LCM）的形态、粒径及其分布、zeta电位以及声学性质做了测定。研究了该微泡在不同浓度下的散射特性和二次谐波散射性质, 并分析了浓度和散射波幅度之间的关系。考察了LCM体外增强血栓显影的效果。结果该微泡的平均粒径为3.38 μm, 95％的微泡粒径小于5 μm。声学性质表明有造影剂的情况下, 在不同的实验浓度下均能接收到收发探头分置且两探头成90º方式下的散射波, 二次谐波的散射强度随着微泡浓度的降低逐渐降低, 并且由微泡产生的二次谐波相对生理盐水空白产生的谐波幅度的增加率和微泡浓度成线性关系。LCM能显著增强超声显像效果, 使得血栓显影更加清晰。结论该自制微泡表现出了良好的物理状态、声学性质以及造影增强效果。

关键词: 微泡, 微泡, 微泡, 超声造影剂, 超声造影剂, 超声造影剂, 造影, 造影, 造影, 声学性质, 声学性质, 声学性质, 散射, 散射, 散射, 二次谐波, 二次谐波, 二次谐波

Abstract:

Aim To research on a stable microbubble with good acoustic properties and excellent imaging enhancing effect, and to make it to be a promising agent for the enhancement of ultrasound imaging in the ultrasound diagnosis of cardiovascular system diseases. Methods The morphology, size and zeta potential of lipid-coated microbubbles (LCM), the acoustic properties of backscatter, and the second harmonic scatter of LCM were determined. Furthermore, the relationship between the concentrations and the amplitude values of the second harmonic was investigated by testing the intensities of the second harmonic at different concentrations. The imaging effect of LCM was also studied in vitro. Results The mean diameter of LCM was 3.38 μm with 95% of the bubbles under 5 μm. The scatter signal generated by microbubbles was observed under different concentrations in the bistatic modes while the position of transmitting transducer and receiving transducer was orthogonal. The intensity of the second harmonic scatter fell with the decrease of microbubble concentration. The increase rate of the second harmonic amplitude values generated by the microbubbles versus that by physiological saline was linear with the natural logarithm of bubble concentrations. The LCM could enhance the ultrasound image of thrombus. Conclusion The LCM exhibited good physical state and acoustic properties, which could increase the imaging quality.

Key words: Microbubble, Microbubble, Ultrasound contrast agent, Ultrasound contrast agent, Imaging, Imaging, Acoustic property, Acoustic property, Scatter, Scatter, The second harmonic, The second harmonic

中图分类号:

R943

Supporting: ^*Corresponding author. Tel.: 86-10-82801584; fax: 86-10-82802791

向宇, 孙剑, 齐宪荣^*.

脂质包膜微泡超声造影剂基础物理和声学性质以及体外造影增强实验

[J]. .

Yu Xiang, Jian Sun, Xian-Rong Qi^*. Basic physical, acoustic properties, and in vitro ultrasound imaging enhancement of the lipid-coated microbubbles[J]. .

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