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HPLC法测定一种新型鬼臼毒素衍生物脂质体的包封率

向蓉, 陈世武, 张辅民, 倪京满*, 田瑄   

  1. 1. 兰州大学 药学院, 甘肃 兰州 730000
    2. 兰州中医骨伤科医院 药剂科, 甘肃 兰州 730000
    3. 兰州大学 功能有机分子化学国家重点实验室, 甘肃 兰州 730000

  • 收稿日期:2009-01-05 修回日期:2009-05-10 出版日期:2009-06-15 发布日期:2009-06-15
  • 通讯作者: 倪京满*

A HPLC method for the determination of entrapment efficiency of a new
5-FPE liposome formulation

Rong Xiang, Shi-Wu Chen, Fu-Min Zhang, Jing-Man Ni*, Xuan Tian   

  1. 1. School of Pharmacy, Lanzhou University, Lanzhou 730000, China;
    2. Department of Pharmacy, Lanzhou Hospital of Traditional Chinese Medicine Traumatocomium, Lanzhou 730000, China;
    3. Sates Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China
  • Received:2009-01-05 Revised:2009-05-10 Online:2009-06-15 Published:2009-06-15
  • Contact: Jing-Man Ni*

摘要:

建立采用高效液相色谱法测定5-FPE脂质体包封率的方法。色谱柱为Kromasil 100 A C18 column (350 mm×4.6 mm, 5 μm), 流动相为甲醇-水(58:42, v/v), 流速为0.8 mL/min, 测定波长为271 nm, 进样量为10 µL, 柱温为30 ºC。线性范围为0.8~12.8 µg/mL, 相关系数 r = 0.9999, 日内和日间精密度的RSD小于2.97%, 平均回收率为96.8%~104.6%, RSD小于2.24%。本文建立了采用高效液相色谱测定5-FPE脂质体包封率的方法, 该方法简便、快速、精确、灵敏度高。

关键词: 鬼臼毒素, 高效液相色谱, 包封率, 脂质体

Abstract:

A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation. Chromatographic separation was performed on a Kromasil 100 A C18 column (350 mm×4.6 mm, 5 μm). The mobile phase was consisted of methanol-water (58:42, v/v). The flow rate of mobile phase was set at 0.8 mL/min. The UV detection wavelength was 271 nm, and the column temperature was 30 ºC. The linear range of 5-FPE was from 0.8-12.8 µg/mL, r = 0.9999. The RSD of intra-day and inter-day precision were less than 2.97%. The average recovery was from 96.8%-104.6% with RSD less than 2.24%. The method was simple, rapid, accurate, and sensitive. It is suitable for the determination of entrapment efficiency of the 5-FPE liposome formulation.

Key words: Podophyllotoxin, Podophyllotoxin, HPLC, HPLC, Entrapment efficiency, Entrapment efficiency, Liposome, Liposome

中图分类号: 

Supporting: Foundation items: Natural Science Foundation of Gansu Provence (Grant No. 0803RJZA079), Foundation of Herb Medicin Research of Gansu Provence (Grant No. GZK-2009-1).

*Corresponding author. Tel.: 13088775264;
e-mail: lynjm2004@hotmail.com