苄基四氢巴马汀和甲氧胺对豚鼠心室肌细胞延迟整流钾电流阻滞作用

苄基四氢巴马汀和甲氧胺对豚鼠心室肌细胞延迟整流钾电流阻滞作用

李泱^*, 傅丽英, 姚伟星, 夏国瑾, 江明性

1.华中科技大学同济医学院心内科, 武汉 430030;
2.华中科技大学同济医学院药理教研室, 武汉 430030

收稿日期:2002-05-18 修回日期:2002-06-05 出版日期:2002-09-15 发布日期:2002-09-15
通讯作者: 李泱*

Effects of Benzyltetrahydropalmatine and Methoximine on Delayed Rectifier Potassium Channel Current in Guinea Pig Ventricular Myocytes

Li Yang^*, Fu Liying, Yao Weixing, Xia Guojin, Jiang Mingxing

1.Department of Cardiology, Tongji Hospital,Wnhan 430030;
2.Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030

Received:2002-05-18 Revised:2002-06-05 Online:2002-09-15 Published:2002-09-15
Contact: Li Yang*

摘要/Abstract

摘要：

目的研究苄基四氢巴马汀(BTHP)和甲氧胺对豚鼠心室肌细胞上延迟整流钾电流作用. 方法采用全细胞膜片钳技术测定延迟整流钾电流(Ik). 结果甲氧胺50μmol·L^-1在37°C时可以分别使Ik和Ik,tail 从(12.4±1.8)pA pF^-1和(4.2±0.5)pA pF^-1上升至(20.5±0.7)pA pF^-1和(7.2±0.6)pA pF^-1. 若事先甲氧胺50μmol·L^-1后, 再加入BTHP 30μmol·L^-1可使BTHP阻滞Ik和Ik,tail百分率分别从单独使用BTHP时的37.5%±6.0%和35.9±5.5%上升到62.9%±4.3%和61.1%±3.7%. 结论苄基四氢巴马汀可以逆转甲氧胺对豚鼠心室肌细胞上IK的增加作用.

关键词: 苄基四氢巴马汀, 甲氧胺, 延迟整流钾电流, 膜片钳, 心肌细胞

Abstract: Objactive To investigate the effects of benzyltetrahydropalmatine and methoximine on delayed rectifier potassium current (Ik) in guinea pig ventricular myocytes. Methods Whole-cell patch clamp technique was used to record Ik current. Results Methoximine 50 μmol·L^-1 augmented densities of Ik and Ik,tail from (12.4±1.8)pA pF^-1 and (4.2±0.5 )pA pF^-1 to (20.5±0.7)pA pF^-1 and (7.2±0.6)pA pF^-1 respectively. The effect of methoximine on the Ik and Ik,tail can be reversed by BTHP 30 μmol·L^-1, with the inhibition rates of 62.9%±4.3% and 61.1%±3.7%, respectively. When the cells were exposed only to 30 μmol·L^-1 BTHP, Ik and Ik,tail were blocked by 37.5%±6.0% and 35.9%±5.5%. Conclusion The results show that BTHP can inhibit the enhancing effect of methoximine on Ik in guinea pig ventricular myocytes.

Key words: Benzyltetrahydropalmatine, Benzyltetrahydropalmatine, Methoximine, Methoximine, Delayed rectifier potassium current, Delayed rectifier potassium current, Patch-clamp technique, Patch-clamp technique, Myocytes, Myocytes

Supporting: ^*Foundation item: Natural Science Foundation of Hubei Province (99J154).
Corresponding author Tel: (027) 83663545, E-mail: liyang40@hotmail.com

李泱^*, 傅丽英, 姚伟星, 夏国瑾, 江明性. 苄基四氢巴马汀和甲氧胺对豚鼠心室肌细胞延迟整流钾电流阻滞作用[J]. .

Li Yang^*, Fu Liying, Yao Weixing, Xia Guojin, Jiang Mingxing . Effects of Benzyltetrahydropalmatine and Methoximine on Delayed Rectifier Potassium Channel Current in Guinea Pig Ventricular Myocytes[J]. .

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