穿龙薯蓣总皂苷中甾体皂苷的分离与鉴定

穿龙薯蓣总皂苷中甾体皂苷的分离与鉴定

都述虎^*, 刘文英, 付铁军, 李伯刚, 夏重道

1.安徽省医学科学研究所,合肥230061;
2.中国药科大学药物分析教研室,南京210009;
3.中国科学院成都地奥制药公司, 成都610041

收稿日期:2002-03-29 修回日期:2002-07-06 出版日期:2002-09-15 发布日期:2002-09-15
通讯作者: 都述虎*

Isolation and Identification of Steroidal Sapogenins from Total Sapogenin of Dioscorea Nipponica Makino

Du Shuhu^*, Liu Wenying, Fu Tiejun , Li Bogang, Xia Chongdao

1.Anhui Institute of Medical Sciences, Hefei 230061;
2.Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009;
3.Chengdu Di'Ao Pharmaceutical Company of Academia Sinica, Chengdu 610041

Received:2002-03-29 Revised:2002-07-06 Online:2002-09-15 Published:2002-09-15
Contact: Du Shuhu*

摘要/Abstract

摘要： 目的研究穿龙薯蓣总皂苷中水溶性甾体皂苷, 寻找新的活性化合物. 方法用硅胶柱色谱、薄层色谱及高效液相色谱等进行分离, 通过酸水解、理化常数和波谱学分析(IR, NMR, MS, HMQC, HMBC) 鉴定化合物结构. 结果从穿龙薯蓣总皂苷中分得2个甾体皂苷(1个水难溶性皂苷和1个水溶性皂苷), 其化学结构分别鉴定为薯蓣皂苷元-3-o{α-L-鼠李糖(1→2)-[β-D-葡萄糖(1→3)]}-β-D-葡萄糖皂苷(I), 薯蓣皂苷元-3-O-[a-L-鼠李糖(1→3)-a-L-鼠李糖(1→4)-a-L-鼠李糖(1→4)]-β-D-葡萄糖皂苷(II). 结论 II为首次从穿龙薯蓣中分离得到的新化合物, 命名为穿龙薯蓣皂苷Dc.

关键词: 穿龙薯蓣总皂苷, 甾体皂苷, 穿龙薯蓣皂苷Dc

Abstract: Aim To investigate the water-soluble steroidal sapogenins from total sapogenins of Dioscorea nipponica Makino and to look for new active compounds. Methods The compounds were isolated with silica gel, PTLC and HPLC,and their structures were elucidated by acid hydrolysis, physical and chemical data and spectroscopic analyses (IR, NMR,MS, HMQC, HMBC) as well as chemical correlations. Results The two steroidal sapogenins (water-insoluble sapogenin and water-soluble sapogenin) were isolated from the total sapogenins of Dioscorea nipponica Makino. The structures were elucidated as diosgenin 3-O-{α-L-rhamnopyranosyl (1→2)-[β-D-glucopyranosyl (1→3)]}-β-D-glucopyranoside (I), diosgenin 3-O-[α-L-rhamnopyranosyl(1→3)-α-L-rhamnopyranosyl (1→4)-α-L-rhamnopyranosyl (1→4) ]-β-D-glucopyranoside (II). Conchusion Compound II is a new steroidal sapogenin and has been found in Dioscorea nipponica Makino for the first time . It was named dioscin Dc.

Key words: Total sapogenins of Dioscorea nipponica Makino, Total sapogenins of Dioscorea nipponica Makino, Steroidal sapogenins, Steroidal sapogenins, Dioscin Dc, Dioscin Dc

Supporting:

^*First published in Chinese in Acta Pharmaceutica Sinica. 2002, 37 (4): 267.
Spported by the Anhui Provincial Outstanding Young Science Fund (200102-23), the New Drug Fund of Chengdu Di Ao Pharmaceutical Company (991101).
Biographies: Du Shuhu (1962-) ,male , associate research fellow, Ph. D Candidate.
Corresponding author. Tel.: (0551) 2828461; Fax: (0551) 2825823; E-mail: Shuhudu1@yahoo.com.cn

都述虎^*, 刘文英, 付铁军, 李伯刚, 夏重道. 穿龙薯蓣总皂苷中甾体皂苷的分离与鉴定[J]. .

Du Shuhu^*, Liu Wenying, Fu Tiejun , Li Bogang, Xia Chongdao. Isolation and Identification of Steroidal Sapogenins from Total Sapogenin of Dioscorea Nipponica Makino[J]. .

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