RP-HPLC法测定青娥丸提取物中松脂醇二葡萄糖苷含量

RP-HPLC法测定青娥丸提取物中松脂醇二葡萄糖苷含量

熊志立, 罗璇, 李小芩, 李发美^*

沈阳药科大学药学院, 沈阳 110016

收稿日期:2004-02-11 修回日期:2004-05-10 出版日期:2004-06-15 发布日期:2004-06-15
通讯作者: 李发美*

RP-HPLC Determination of Pinoresinol Diglucopyranoside in Qing'e Pill Extract

XIONG Zhi-li, LUO Xuan, LI Xiao-qin, LI Fa-mei^*

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China

Received:2004-02-11 Revised:2004-05-10 Online:2004-06-15 Published:2004-06-15
Contact: LI Fa-mei*

摘要/Abstract

摘要： 目的建立测定青娥丸提取物中松脂醇二葡萄糖苷含量的RP-HPLC法.方法青娥丸提取物用75%乙醇提取, 经AB-8大孔吸附树脂纯化后, 注入高效液相色谱仪. 本法采用ODS分析柱为固定相, 以甲醇-乙腈-水(24:3:78,V/V/V)为流动相, 流速为1.0 mL·min^-1, 紫外检测波长为227 nm. 结果松脂醇二葡萄糖苷的线性范围为5.5-170 μg·mL^-1 (r>0.9998),平均回收率99.3%. 日内和日间精密度分别为1.3%和2.8%(n=5). 青娥丸中松脂醇二葡萄糖苷的含量为0.446±0.012 mg·g^-1 (n=10). 结论本方法灵敏、准确, 专属性强, 适用于青娥丸及其他复方中药中松脂醇二葡萄糖苷的含量测定.

关键词: 反相高效液相色谱法, 反相高效液相色谱法, 松脂醇二葡萄糖苷, 松脂醇二葡萄糖苷, 青娥丸, 青娥丸

Abstract: Aim To develop and determine pinoresinol diglucopyranoside in Qing’e Pill, a traditional Chinese com-pound preparation containing Eucommia ulmoides Oliv. as the principal drug, by a reverse-phase high-performance liquid chromatographic method (RP-HPLC). Methods The extract of Qing'e Pill was refiuxed with 75% ethanol, purified on an AB-8 macroporous adsorption resin column and then injected into HPLC system. The HPLC assay was performed on an ODS analytical column with a mixture of methanol-acetonitrile-water (24:3:78, V/V/V) as the mobile phase at a flow-rate of 1.0 mL·min^-1, and a UV detector set at 227 nm. Results Good linearity between peak area and concentration was found in the range of 5.5-170 μg·mL^-1 for pinoresinol diglucopyranoside ( r>0.9998). The average recovery was 99.3%. The intra-day assay RSD and the inter-day assay RSD were 1.3% and 2.8%, respectively (n = 5). The content of pinoresinol diglucopyranoside in Qing'ePillwasdetermined to be 0.446±0.012mg·g^-1 (n = 10). Conclusion The RP-HPLC meth-od was proved to be sensitive, specific, accurate and precise for the determination of pinoresinol diglucopyranoside in Qing’e Pill.

Key words: RP-HPLC, RP-HPLC, pinoresinol diglucopyranoside, pinoresinol diglucopyranoside, Qing'e Pill, Qing'e Pill

中图分类号:

R917.01

R927.2

Supporting:

^*Corresponding author. Tel.: 024-23843711-3361; fax: 024-83890024

熊志立, 罗璇, 李小芩, 李发美^*. RP-HPLC法测定青娥丸提取物中松脂醇二葡萄糖苷含量[J]. .

XIONG Zhi-li, LUO Xuan, LI Xiao-qin, LI Fa-mei^*. RP-HPLC Determination of Pinoresinol Diglucopyranoside in Qing'e Pill Extract[J]. .

[1]	李馨儒, 李艳芳, 周艳霞, 李晓燕, 刘艳^*. RP-HPLC 法测定微乳中水飞蓟素的含量[J]. , 2009, 18(2): 190-192.
[2]	刘祥瑞, 吴科春, 张春晖, 张烜^*, 张强 . 反相高效液相色谱法测定紫杉醇固体分散体中紫杉醇的含量[J]. , 2007, 16(2): 101-104.
[3]	卢定强^*, 陆晓云, 陆凯丰, 胡继军. RP-HPLC测定番茄红素微胶囊的含量[J]. , 2007, 16(1): 65-68.
[4]	王会娟, 李馨儒, 黄燕清, 刘艳^*. 反相高效液相色谱法测定狗血浆中番茄红素的浓度[J]. , 2004, 13(4): 259-261.
[5]	孟湘明, 孟志云, 张亮, 窦桂芳^*. RP-HPLC法同时测定注射用粉末中他唑巴坦和头孢他啶组分的含量[J]. , 2004, 13(4): 267-270.
[6]	仇峰, 唐星, 何仲贵^*, 李好枝. 黄芩苷水溶液的稳定性[J]. , 2004, 13(2): 134-137.
[7]	李素华, 蒋学华^*, 兰轲, 杨俊毅, 周静. 灯盏乙素在家犬体内的药动学研究[J]. , 2003, 12(3): 127-130.
[8]	李文彦, 毕开顺^*, 乔延江, 宁黎丽, 罗旭. 贝母中浙贝甲素和浙贝乙素的柱前衍生化高效液相色谱测定[J]. , 1996, 5(4): 213-218.