从两株微小亚历山大藻中分离纯化膝沟藻毒素

从两株微小亚历山大藻中分离纯化膝沟藻毒素

缪宇平, 陈逸民, 周宏农, 闻韧^*

1.中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090; 2.复旦大学药学院,上海200032; 台湾大学理学院, 台北10617;
3.台湾大学理学院,台北10617

收稿日期:2003-08-29 修回日期:2004-05-10 出版日期:2004-06-15 发布日期:2004-06-15
通讯作者: 闻韧*

Isolation and Purification of Gonyautoxins from Two Strain of Alexandrium minutum Halim

MIAO Yu-ping, CHEN Yi-min, ZHOU Hong-nong, WEN Ren^*

1.Key and Open Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;
2.School of Pharmacy, Fudan University, Shanghai 200032, China;
3.Institute of Fisheries Science, National Taiwan University, Taibei 10167, China

Received:2003-08-29 Revised:2004-05-10 Online:2004-06-15 Published:2004-06-15
Contact: WEN Ren*

摘要/Abstract

摘要： 目的　从两株微小亚历山大藻(Alexandrium minutum Halim)中分离纯化膝沟藻毒素(Gonyautoxins)。方法　用胶滤层析、离子交换层析等方法从人工培养的两株微小亚历山大藻酸酒精萃取物中分离纯化膝沟藻毒素,再用HPLC法对毒素进行定性定量测定。结果　从两株藻株培养液中均分离得到膝沟藻毒素GTX-4, GTX-1, GTX-3, GTX-2, 其中Amtk4藻株的毒素含量为Amtk2的近10倍。结论　微小亚历山大藻Amtk4藻株适于制备膝沟藻毒素。

关键词: 微小亚历山大藻, 微小亚历山大藻, 微小亚历山大藻, 膝沟藻毒素, 膝沟藻毒素, 膝沟藻毒素, 胶滤层析, 胶滤层析, 胶滤层析, 离子交换层析, 离子交换层析, 离子交换层析

Abstract: Aim To study isolation and purification of Gonyautoxins from two different strains of Alexandrium minutum Halim. Methods Ethanol extracts of the two culture strains of Alexandrium minutum Halim were fractionated by gel filtration and ion exchange chromatography to obtain gonyautoxins. The toxin profiles were tested by HPLC method. Results Both strains contain gonyautoxin-1, gonyautoxin-2, gonyautoxin-3 and gonyautoxin-4. The total gonyautoxin content of Amtk4 strain is almost 10 times higher than that of Amtk2 strain. Conclusion Alexandrium minutum Halim Amtk4 strain is suitable for the preparation of Gonyautoxins.

Key words: Alexandrium minutum Halim, Alexandrium minutum Halim, gonyautoxins, gonyautoxins, gel filtration chromatography, gel filtration chromatography, ion exchange chromatography, ion exchange chromatography

中图分类号:

R915.1

R931.711

Supporting: ^*Corresponding author. Tel.: 021-54237560

缪宇平, 陈逸民, 周宏农, 闻韧^*. 从两株微小亚历山大藻中分离纯化膝沟藻毒素[J]. .

MIAO Yu-ping, CHEN Yi-min, ZHOU Hong-nong, WEN Ren^*. Isolation and Purification of Gonyautoxins from Two Strain of Alexandrium minutum Halim[J]. .