离子化修饰当归多糖衍生物的形貌及体外抗氧化活性研究

doi:10.5246/jcps.2024.04.024

中国药学(英文版) ›› 2024, Vol. 33 ›› Issue (4): 316-328.DOI: 10.5246/jcps.2024.04.024

离子化修饰当归多糖衍生物的形貌及体外抗氧化活性研究

王婕, 黄子贞, 薛伟娇, 张妍, 谈春霞^*()

甘肃中医药大学, 甘肃兰州 730000

收稿日期:2023-09-25 修回日期:2023-10-08 接受日期:2023-11-10 出版日期:2024-04-30 发布日期:2024-04-30
通讯作者: 谈春霞

The morphology and in vitro antioxidative effect of ionized polysaccharide from Angelica Sinensis

Jie Wang, Zizhen Huang, Weijiao Xue, Yan Zhang, Chunxia Tan^*()

Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu, China

Received:2023-09-25 Revised:2023-10-08 Accepted:2023-11-10 Online:2024-04-30 Published:2024-04-30
Contact: Chunxia Tan
Supported by:
Project of Administration of Tradition of Chinese Medicine of Gansu Province (Grant No. GZKP-2021-35); Gansu University of Traditional Chinese Medicine Scientific Research and Innovation Fund (Grant No. 2021KCZD-3); Gansu Provincial Science and Technology Plan Funded Natural Science Fund Project (Grant No. 22JR11RA119).

摘要/Abstract

摘要：

本文旨在研究离子化修饰对当归多糖的形貌及体外抗氧化活性的影响。以当归多糖(ASP)为原料, 混合磷酸盐(磷酸二氢钠和磷酸氢二钠)和3-氯-2-羟丙基三甲基氯化铵(CHPTAC)为改性剂, 分别对其进行磷酸化、季铵化修饰, 得到磷酸化当归多糖(ASP-P)和阳离子化当归多糖(ASP-N), 并测定当归多糖的磷酸化和阳离子化的取代度。通过红外光谱、刚果红实验、扫描电镜及热重分析等对离子化当归多糖衍生物进行形貌表征和热稳定性测试。采用羟基自由基、ABTS自由基及DPPH自由基的清除实验, 评估离子化修饰前后当归多糖的体外抗氧化能力变化。离子化修饰后的当归多糖具有更好的热稳定性, 其中经过磷酸化修饰后的当归多糖热稳定性最好。溶液中离子化修饰的ASP-P/ASP-N保持了ASP的三螺旋骨架构象, 但是在固态时呈现较大尺寸的堆积或卷曲的片状形貌。当归多糖的离子修饰产物可显著提高当归多糖对羟基自由基和ABTS自由基的清除能力, 具有一定的体外抗氧化能力。上述实验结果为进一步研究当归多糖的衍生化提供了实验依据。

关键词: 离子化修饰, 当归多糖, 形貌, 热稳定性, 体外抗氧化活性

Abstract:

abstract

Key words: Ionization modification, Angelica Sinensis polysaccharide, Morphology, Thermal stability, Antioxidant activity in vitro

Supporting:

王婕, 黄子贞, 薛伟娇, 张妍, 谈春霞. 离子化修饰当归多糖衍生物的形貌及体外抗氧化活性研究[J]. 中国药学(英文版), 2024, 33(4): 316-328.

Jie Wang, Zizhen Huang, Weijiao Xue, Yan Zhang, Chunxia Tan. The morphology and in vitro antioxidative effect of ionized polysaccharide from Angelica Sinensis[J]. Journal of Chinese Pharmaceutical Sciences, 2024, 33(4): 316-328.

图/表 8

Figure 1. Schematic diagram of preparation ASP-P and ASP-N from ASP.

Figure 2. IR spectra of ASP, ASP-P and ASP-N.

Figure 3. Thermogravimetric analysis curves of ASP, ASP-P and ASP-N.

Figure 4. The change curve of λmax based on interaction of Congo-red with ASP, ASP-P and ASP-N.

Figure 5. The XRD of ASP, ASP-P and ASP-N.

Figure 6. The DLS curve of ASP-P and ASP-N.

Figure 7. SEM images of ionized ASP. (a) ASP (5 kV, 5000×); (b) ASP-P (5 kV, 5000×) and (c) ASP-N (5 kV, 2000×).

Figure 8. Antioxidant activity analysis of ASP, ASP-P and ASP-N. (a) Hydroxyl radical scavenging assay; (b) ABTS radical scavening assay; (c) DPPH radical scavenging assay (*P < 0.05, **P < 0.01, ***P < 0.001, No Significant).

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离子化修饰当归多糖衍生物的形貌及体外抗氧化活性研究

The morphology and in vitro antioxidative effect of ionized polysaccharide from Angelica Sinensis

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