功能性L-Cys-CdS纳米粒子共振光散射法测定脱氧核糖核酸

功能性L-Cys-CdS纳米粒子共振光散射法测定脱氧核糖核酸

戴美玲, 严拯宇^*, 曲萍, 邵秀芬

中国药科大学分析化学系, 江苏南京 210038

收稿日期:2005-10-25 修回日期:2006-05-10 出版日期:2006-06-15 发布日期:2006-06-15
通讯作者: 严拯宇*

Application of L-Cys-Capped CdS Nanoparticles in Determination of Nucleic Acid by Resonance Light-Scattering Technique

DAI Mei-ling, YAN Zheng-yu^*, QU Ping, SHAO Xiu-fen

Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210038, China

Received:2005-10-25 Revised:2006-05-10 Online:2006-06-15 Published:2006-06-15
Contact: YAN Zheng-yu*

摘要/Abstract

摘要： 目的报道了功能性L-Cys-CdS纳米荧光探针的合成, 纳米粒子水溶性好, 稳定, 且具有较好的荧光特性。方法以该纳米粒子为荧光探针, 探讨了DNA对其的荧光猝灭作用。结果表明在Tris-HCL溶液中DNA对CdS的RLS有很好的猝灭, 小牛胸腺DNA及鱼精DNA的线性范围及检测限分别为0.01-1.0 μg·mL^-1, 8 ng·mL^-1; 0.04-1.5 μg·mL^-1, 10 ng·mL^-1。结论该方法简便、快速、灵敏, 用于测定合成样品取得了满意的结果。

关键词: CdS, CdS, CdS, L-半胱氨酸, L-半胱氨酸, L-半胱氨酸, 共振光散射, 共振光散射, 共振光散射, DNA, DNA, DNA

Abstract: Aim To determine nucleic acid (DNA) using Nanometer-sized L-cysteine-capped CdS particles by resonance light scattering (RLS) method. Methods The nano-particles synthesized by a colloidal aqueous method were water-soluble, stable, and highly luminescent. The RLS of L-Cys-CdS particles were greatly quenched by DNA in Tris-HCl solutions. The intensity of RLS at 344 nm was proportional to the concentration of DNA. Results The linearity range of the calibration curve was 0.01-1.0 μg·mL^-1 for calf thymus DNA and 0.04-1.5 μg·mL^-1 for salmon sperm DNA. The detection limits (3 δ) were 8 ng·mL^-1 for calf thymus DNA and 10 ng·mL^-1 for salmon sperm DNA. Conclusion This method is simple, sensitive, and capable of avoiding the use of toxic dyes.

Key words: CdS, CdS, resonance light scattering, resonance light scattering, cysteine, cysteine, DNA, DNA

中图分类号:

R917.02

R917.797

Supporting:

Foundation item: National Natural Science Foundation of China (30370404).
^*Corresponding author. Tel.: 13512503905

戴美玲, 严拯宇^*, 曲萍, 邵秀芬. 功能性L-Cys-CdS纳米粒子共振光散射法测定脱氧核糖核酸[J]. .

DAI Mei-ling, YAN Zheng-yu^*, QU Ping, SHAO Xiu-fen. Application of L-Cys-Capped CdS Nanoparticles in Determination of Nucleic Acid by Resonance Light-Scattering Technique[J]. .

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