羧基化PEG修饰的磁流体的制备和表征

doi:10.5246/jcps.2012.01.006

羧基化PEG修饰的磁流体的制备和表征

周彦菲, 杜玲然, 周超, 范田园^*

北京大学医学部药学院药剂学系, 北京 100191

收稿日期:2011-08-19 修回日期:2011-10-19 出版日期:2012-01-01 发布日期:2012-01-01
通讯作者: 范田园*

Preparation and characterization of ferromagnetic fluids modified by carboxyl PEG

Yan-Fei Zhou, Ling-Ran Du, Chao Zhou, Tian-Yuan Fan^*

Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2011-08-19 Revised:2011-10-19 Online:2012-01-01 Published:2012-01-01
Contact: Tian-Yuan Fan*

摘要/Abstract

摘要： 制备和表征肿瘤热疗用羧基化PEG修饰的Fe3O4磁流体。化学共沉淀方法制备四氧化三铁磁性纳米颗粒, 然后用羧基化PEG修饰; 用邻二氮菲显色法测定磁流体中铁的含量; 沉降方法考察了制备的磁流体的稳定性; 通过X射线衍射、透射电子显微镜、红外和振动样品磁强计对制备的磁流体进行表征; 测定了磁流体在交变磁场作用下的热效应。羧基化PEG修饰的磁流体稳定性明显优于未修饰的; 红外图谱和X衍射图谱证明所制备的磁流体样品由Fe3O4组成; 透射电镜照片显示磁性粒子分散良好; 经X衍射数据计算得磁性粒子的粒径约为5 nm; 羧基化PEG修饰磁流体的饱和磁化强度和剩余磁化强度分别为47.01和3.41 emu/g。矫顽力为6.7 Oe; 磁流体的特征吸收率为63.0 W/g[Fe]。羧基化PEG修饰的Fe3O4磁流体有望用于肿瘤热疗。

关键词: 铁磁流体, 四氧化三铁, 羧基化聚乙二醇, 热效应, 热疗

Abstract:

To prepare and characterize the ferromagnetic fluid of Fe3O4modified by carboxyl PEG (FF/carboxyl PEG) for hyperthermia of tumor, the magnetic nanoparticles (NPs) of Fe3O4were prepared by chemical co-precipitation method, and then modified with carboxyl PEG. The iron content of FFs was determined by spectrophotometric method using o-phenanthroline. The stability of FF/carboxyl PEG was assessed by the sedimentation method. FF/carboxyl PEG was characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), infrared spectrometry (IR) and vibrating sample magnetometer (VSM). Heating effect of FF/carboxyl PEG was measured in an alternating magnetic field in vitro. The stability of FF/carboxyl PEG was much better than that of unmodified ferromagnetic fluid. FF/carboxyl PEG was proved to be composed of Fe3O4 by both XRD and IR. TEM showed that the ferromagnetic particles were well-dispersed. The average particle size was calculated as 5 nm by XRD. The saturation magnetization and residual magnetization of FF/carboxyl PEG were 47.01 and 3.41 emu/g, respectively. The coercive force was 6.7 Oe. The specific absorption rate (SAR) of the FF/carboxyl PEG was 63.0 W/g[Fe]. The FF/carboxyl PEG shows the promise for hyperthermia of tumor.

Key words: Ferromagnetic fluid, Fe3O4, Carboxyl PEG, Heating effect, Hyperthermia

中图分类号:

Supporting: ^*Corresponding author. Tel.: 86-10-82801584

周彦菲, 杜玲然, 周超, 范田园^*. 羧基化PEG修饰的磁流体的制备和表征[J]. , DOI: 10.5246/jcps.2012.01.006.

Yan-Fei Zhou, Ling-Ran Du, Chao Zhou, Tian-Yuan Fan^*. Preparation and characterization of ferromagnetic fluids modified by carboxyl PEG[J]. , DOI: 10.5246/jcps.2012.01.006.

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