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Table of Content

    08 May 2013, Volume 22 Issue 3
    Contents

    Graphical contents list

    Journal of Chinese Pharmaceutical Sciences

    2013, 22(3):  205-208. 
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    Reviews
    Recent advances in the chemical and biological studies of Aconitum pharmaceutical resources
    Dacheng Hao*, Xiaojie Gu, Peigen Xiao*, Lijia Xu, Yong Peng
    2013, 22(3):  209-221.  DOI: 10.5246/jcps.2013.03.030
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    Many herbal medicines have so far been utilized for the treatment of various ailments since the beginning of Chinese civilization. Aconitum is a botanical source for various pharmaceutically active components, which has been commonly used in traditional Chinese medicine for thousands of years. Increasing interest in Aconitum pharmaceutical resources has led to further discoveries of diterpenoid alkaloids, polysaccharides, flavonoids and many other compounds in various Aconitum species, and to investigations on their chemotaxonomy, molecular phylogeny and pharmacology. In continuation with our studies on Aconitum pharmacophylogeny, here we review the phytochemistry, chemotaxonomy, molecular biology and phylogeny of Aconitum and their relevance to therapeutic efficacy and toxicity. An exhaustive literature survey is used to summarize the global scientific effort in the phytochemical and biological studies of Aconitum. More diterpenoid alkaloids have been found in various Aconitum species, among which the aconitine type (type III) is predominant. The versatile bioactivities of alkaloids and extracts, as well as the bioactivities of polysaccharides and other ingredients, are summarized and discussed in this review. The morphology-based 11-series classification of section Aconitum, subgenus Aconitum, is not supported by chemotaxonomy and molecular phylogeny. Molecular phylogeny based on nuclear and chloroplast DNA sequences divided the nine morphologically similar series into two clusters, which is bolstered by the chemotaxonomic data. It is essential to integrate the emerging technologies into Aconitum studies for both the sustainable utilization of Aconitum pharmaceutical resources and finding novel compounds with potential clinical utility and less toxicity. Systems biology and omics technologies will play an increasingly important role in booming pharmaceutical research involving bioactive compounds of Aconitum.
    Cardiovascular protective effects of astragaloside IV
    Shuai Liu, Jinguo Zhang*
    2013, 22(3):  222-225.  DOI: 10.5246/jcps.2013.03.031
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    Astragali Radix has been widely used in traditional Chinese medicine for centuries, especially for the treatment of cardiovascular diseases. Astragaloside IV (AST IV), one of the main active ingredients in Astragali Radix, is involved in various functions, including vasodilating effect, protecting the vascular endothelial cells, anti-inflammatory effect, antivirus effect, positive inotropism and so on. These functions are attributed to the ability of AST IV in scavenging oxygen free radicals, the regulation of calcium homeostasis, its anti-oxidative effect and prevention of mitochondrial damage. Astragaloside IV has been effectively used to treat coronary artery disease, heart failure, viral myocarditis, hypertension and other cardiovascular diseases. Its cardiovascular protective effects and mechanisms are worth studying.
    Original articles
    Simultaneous determination of steroidal saponins in Anemarrhena asphodeloides Bge. by ultra high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry
    Yang Zhao, Liping Kang, Heshui Yu, Xu Pang, Zhenfang Wang, Chengqi Xiong, Chao Liu, Lifeng Han, Xiumei Gao, Baiping Ma*
    2013, 22(3):  226-233. 
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    The objective of the research is to develop a quantitative method by ultra high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF MS) for the analysis of seven major steroidal saponins (timosaponin N, timosaponin E1, timosaponin BII, timosaponin B, anemarrhenasaponin I, anemarrhenasaponin A2, and timosaponin AIII) in Anemarrhena asphodeloides Bge. The complete separation of these seven steroidal saponins was achieved within 18 min with an ACQUITY UPLC HSS T3 column using an acetonitrile-water (contain 0.1% formic acid) gradient system. The limits of quantitation (LOQ), 0.18-0.75 ng/µL for seven steroidal saponins, were determined experimentally. The limits of detection (LOD) were found to be 0.05-0.22 ng/µL for these saponins. The correlation coefficients (r2) for calibration curves varied from 0.9902 to 0.9979. This method showed good repeatability for the quantification of these saponins in rhizomes of A. asphodeloides, with intra-day and inter-day variations of less than 5.0% for seven steroidal saponins. The recoveries of seven steroidal saponins were from 97.13% to 101.98%. The validated method was successfully applied to quantifying seven steroidal saponins in various sources of A. asphodeloides (different collecting places or processing methods) and Zhimu concentrate-granules (ZMCG).
    Development of a method for the quantitative determination of geniposide in rat plasma by ......
    Zhipeng Deng, Huixia Fan, Hao Zhong, Shuxiang Cui, Qingqiang Yao*
    2013, 22(3):  234-238.  DOI: 10.5246/jcps.2013.03.033
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    Geniposide is a major bioactive constituent isolated from Gardenia jasminoides Ellis. To evaluate the pharmacokinetics of geniposide in pre-clinical studies, a rapid and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated. After simple protein precipitation, geniposide was analyzed on a Diamonsil® C18 column with a mobile phase of 10 mM ammonium acetate and methanol (20:80, v/v) at a flow rate of 0.6 mL/min. Detection was performed in "Truncated" multiple-reaction monitoring (MRM) mode with positive electrospray ionization (ESI) at m/z 411→411 for geniposide, and MRM mode with negative ESI ionization at m/z 415→295 for puerarin (internal standard, IS). Linearity was established in the concentration range from 10.0 to 5000 ng/mL. The extraction recoveries ranged from 84.8% to 90.5% at concentrations of 10.0, 500 and 4.5×103 ng/mL. The lower limit of quantification (LLOQ) was 10.0 ng/mL with 50 µL plasma. The validated method was successfully applied to the pharmacokinetic study of geniposide in rats at a dose of 200 mg/kg by oral administration.
    Determination of amino acid neurotransmitters in mouse brain tissue using high-performance liquid chromatography with fluorescence detection
    Mengmeng Wang, Wangchun Du, Jie Shen*, Yi Dong, Wenshi Wei, Zhongjuan Song
    2013, 22(3):  239-243.  DOI: 10.5246/jcps.2013.03.034
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    Amino acid neurotransmitters facilitate the transmission of nerve messages across the synapses and play essential roles in the control and regulation of a variety of functions in the central and peripheral nervous system. In this study, we developed a sensitive and efficient method using high-performance liquid chromatography (HPLC) with fluorescence detection for the assay of five important amino acid neurotransmitters. After derivatization with ο-phthaldialdehyde (OPA), aspartate (Asp), glutamic acid (Glu), glycine (Gly), taurine (Tau) and γ-aminobutyric acid (GABA) were simultaneously detected in the presence of the internal standard homoserine (Hse). Precise separation of these five amino acids was achieved using isocratic elution within 24 min. Good linearity was found over the concentration range with correlation coefficients (r2) not less than 0.9998. The limit of detection (LOD) values were no more than 10 nmol/L. The intra- and inter-day reproducibility was adequate with the relative standard deviation (RSD) of 10.5% or below. This method has also been applied to the analysis of amino acids in the substantia nigra and striatum samples obtained from C57BL/6 mice.
    Separation of substituted phenylpiperazine derivatives with immobilized polysaccharide-based chiral stationary phases by supercritical and subcritical fluid chromatography
    Junjun Huang, Mu Yuan*
    2013, 22(3):  244-250.  DOI: 10.5246/jcps.2013.03.035
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    Six newly synthesized racemic 1-(substituted phenyl)-4-[3-(indole-4-yl-oxy)-2-hydroxypropyl]-piperazine 1-6 were successfully resolved by carbon dioxide supercritical fluid chromatography (SFC) on an analytical scale column packed with immobilized polysaccharide-based chiral stationary phases (CSPs). We found that separation on the Chiralpak IA CSP was superior to the other two immobilized CSPs (Chiralpak IB and Chiralpak IC), and isopropanol (IPA) was a superior modifier compared to the other five solvents including ethanol, methanol, tetrahydrofuran, acetonitrile and dichloromethane. The effects of organic modifier composition, back pressure, and column temperature for enantioseparation of all six compounds were studied. Of the physical parameters studied, modifier composition had the greatest impact on retention. Changing temperature generally had less impact on retention but produced the greatest selectivity changes. The optimum condition was found as follows: Chiralpak IA column, column temperature 35 ºC, back pressure 120 bar, 35% IPA containing 0.1% diethylamine (v/v) in mobile phase, flow rate of mobile phase 3.0 mL/min, UV detection 283 nm. Separation of all six racemic compounds was completed within 10 min and excellent resolution was obtained. Thus, SFC was found to be the methodology of choice for resolving the enantiomers of this class of compounds.
    Characteristics, cellular uptake and transepithelial transport of coumarin-6 loaded PEO-(hb-PG)-g-PCL miktoarm copolymer micelles
    Xiaofei Zhang, Zheng Cui, Xiaojin Zhang, Bing He, Xueqing Wang, Hua Zhang, Zhenlin Zhong, Qiang Zhang*
    2013, 22(3):  251-258.  DOI: 10.5246/jcps.2013.03.036
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    Recently, advances in synthetic methodologies have provided efficient routes to generate nonlinear amphiphilic polymers. However, the uptake and transport of model-drug loaded novel miktoarm copolymer micelles have not been extensively studied. In this study, a comparison of the characteristics of two types of PEO-(hb-PG)-g-PCL micelles and the uptake of these two micelles loaded with coumarin-6 (C6) on Caco-2 cellular monolayers was carried out for the first time. PEO113-(hb-PG)15-g-PCL22 micelles (PMs) were then chosen as the vehicle to study the uptake and transport of PMs-C6 across Caco-2 cellular monolayers. In the cellular uptake and transport study, PMs were found to significantly enhance the uptake and transepithelial transport for C6. Mechanism studies further revealed that caveolin-mediated endocytosis pathway, rather than clathrin-mediated pathway, may play an important role in the uptake of PMs-C6. Thus, the novel PEO-(hb-PG)-g-PCL micelles prepared here might have the potential to be used for oral drug delivery due to its advantages in low critical micelle concentration, low cytotoxicity and significant facilitation of the uptake and transport of C6 on Caco-2 cellular monolayers.
    Formulation and evaluation of novel aspirin nanoparticles loaded suppositories
    Ravi Sankar V.*, Dhachinamoorthi D., Chandra Shekar K.B.
    2013, 22(3):  259-267.  DOI: 10.5246/jcps.2013.03.037
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    The main objective of the present work is to design aspirin nanoparticles loaded suppositories which will reduce the side effects caused by aspirin suppositories. Aspirin nanoparticles were prepared initially based on ionic-gelation mechanism and lyophilized. The prepared nanoparticles were evaluated, and the results confirmed that Fa9 formulation was the best with greater drug entrapment efficiency. Aspirin suppositories were prepared in order to investigate the best base composition. The prepared suppositories were evaluated and FS1, FS3, FS4, FS8, FS11, and FS12 were proved to be the best base compositions based on dissolution performed. The lyophilized aspirin nanoparticles of Fa9 were used to prepare aspirin nanoparticles loaded suppositories. The in vitro results revealed that Fas11 was the best formulation.
    Expression, purification and evaluation of N-terminal domain of AcAP5 with Factor Xa inhibitory activity
    Aihua Liu, Yuanjun Zhu, Xiaoyan Liu, Yinye Wang*
    2013, 22(3):  268-271.  DOI: 10.5246/jcps.2013.03.038
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    Ancylostoma anticoagulant peptide 5 (AcAP5) is a strong inhibitor of human coagulation factor Xa (FXa). The N-terminal residues (N40) of AcAP5 contains a domain that could combine with FXa. In order to determine whether N40 protein has FXa inhibitory effect, we cloned, expressed and purified the protein for activity evaluation. The DNA fragment coding N40 was amplified by PCR, cloned into pET-30a to construct recombinant plasmid pET30a-N40, and subsequently transformed into E. coli. BL21 (DE3). Expression of N40 was induced by isopropyl β-D-1-thiogalactopyranoside (IPTG), and the interest protein was identified by SDS-PAGE and purified using one-step nickel (Ni) affinity chromatography. Under the optimal expression condition (0.05 mM IPTG for 6 h at 37 °C), the purity of N40 reached 90%. We also evaluated the inhibition activity of N40 protein on FXa, finding the IC50 was 4.58×10-5 mol/L. This study suggests the N40 of AcAP5 could combine with FXa to inhibit FXa activity.
    Anti-tumor activity of Hedyotis diffusa Willd. in mice
    Yingfeng Wang*, Yi Zheng, Baoshan Ku, Haiyan Yao, Guangyin Yao, Yangli Wan
    2013, 22(3):  272-276.  DOI: 10.5246/jcps.2013.03.039
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    The whole plant with root of Hedyotis diffusa Willd. (AE) has been demonstrated to have anti-inflammatory, antioxidant, and anti-bacterial activities. In our study, we aim to examine the anti-tumor effect of alcoholic extract of AE in mice implanted with sarcoma S180 cells (SBT mice). We also compared the immune system function and the life span of SBT mice with that of control mice. We found that AE displayed a significant inhibitory effect on solid tumor growth, as well as increased the life span. At the dose of 10 mg/kg body weight, both tumor weight and volume were decreased significantly. We also measured several immune function markers, inlcuding spleen index (SI), thymus index (TI) and parameters of hematological. We observed no reduce in these markers, indicating that AE could inhibit tumor growth without affecting immune function in SBT mice.
    A systematic review of hyaluronic acid for burn treatment
    Min Yang, Jing Lu, Xuehua Jiang*
    2013, 22(3):  277-281.  DOI: 10.5246/jcps.2013.03.040
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    To explore the efficiency and safety of hyaluronic acid for burn victims, we searched for all the relative references, selected the references according to the inclusion criterion, assessed them critically and conducted Meta-analysis. The experimental group and the control groups have statistically significant difference in the average time for wound healing. If we make subgroup analysis according to the origin, the outcomes of both foreign study and Chinese study have statistical significance. No statistically significant difference is revealed in the incidence of adverse reaction. In terms of the healing time for diverse burn wounds, statistically significant difference exists in the superficial second-degree burn. There is no statistically significant difference in deep second-degree burn, skin-grafting sites and donor sites. It revealed that hyaluronic acid can accelerate the healing of burn on skin, especially for superficial second-degree burn. And its adverse reaction by external use is negligible.
    Short communications
    Sesquiterpenoids from a South China Sea gorgonian Menella sp.
    Zhiqin Guo, Xingyun Chai, Bin Yang, Xuefeng Zhou, Yonghong Liu*, Pengfei Tu*
    2013, 22(3):  282-285.  DOI: 10.5246/jcps.2013.03.041
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    To investigate the chemical constituents of a South China Sea gorgonian Menella sp., the isolation of ingredients was performed by solvent extraction and repeated chromatography on silica gel, Sephadex LH­20 and preparative TLC. The structures of isolated compounds were determined by spectroscopic analysis and comparison of 1H and 13C NMR data with those reported in literatures. Three subergorane sesquiterpenoids and one suberosane sesquiterpenoid were isolated. Their structures were identified as subergorgic acid (1), subergorgic acid methyl ester (2), 2β-hydroxyl subergorgic acid methyl ester (3), and suberosenol A (4). All compounds were isolated from the genus Menella for the first time.
    Flavanoids and xanthones isolated from Arenaria serpyllifolia L.
    Guanshen Zhou, Xiaojuan Yang, Yong Jiang*, Pengfei Tu
    2013, 22(3):  286-288.  DOI: 10.5246/jcps.2013.03.042
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    Eight known compounds were isolated from the whole plants of Arenaria serpyllifolia L. by solvent fractionation and subsequent separations with silica gel, ODS, Sephadex LH-20 and semi-preparative HPLC chromatography. Their structures were determined by spectroscopic analysis and identified as quercetin (1), quercetin 7-O-α-L-rhamnoside (2), quercetin 3-O-α-L-rhamnoside (3), 3-O-methyl quercetin (4), 1,5-dihydroxyxanthone (5), 1,5-dihydroxyxanthone-6-O-β-D-glucoside (6), 5-hydroxy-1-methoxyxanthone (7) and 6-deoxyisojacareubin (8). All these compounds were isolated from this title plant for the first time, and compounds 5-8 were obtained from genus Arenaria for the first time