Abstract: To search for the protective actions of blumea flavanones (BFs) on hepatocytes and hepatic subcellular organelle against lipid peroxidation, monkey's hepatocytes were isolated and cultured with or without blumea flavanones, then damaged by FeSO4-cysteine or CCl4. The lipid peroxidation (malondialdehyde production) and alteration in hepatocyte membrane (leakage of GPT) were estimated. Hepatic subcellular organelles were also isolated and incubated with or without blumea flavanones, then injured by FeSO4-ascorbate. The generation of malondialdehyde(MDA) was measured. It was found that BFs 10 and 100 μmol·L^-1 inhibited the MDA generation and GPT (glutamic pyruvic transaminase) leakage out of hepatocytes that were induced by CCl4 or FeSO4-cysteine. BFs could prevent lipid peroxidation initiated by FeSO4-ascorbate in subcellular organelle suspension. Among BFs, BF-possessed the strongest activity. Conclusion: Blumea flavanones possess antioxidation activities that protect monkey's hepatocytes and hepatic subcellular organelle against injuries induced by FeSO4 or CCl4.

Key words: Blumea flavanones, Antioxidants, Macaca mulatta, Hepatocyte, Subcellular organelle