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The Effects of Etimicin and Gentamicin on Renal Endoplasmic Reticulum 45Ca2+-Uptake and Ca2+-Mg2+-ATPase Activity

Zhong-Dong Li, Wen-Qing Hong, Pei-Zhong Li   

  1. 1. Department of Clinical Pharmacology, General Hospital of Air Force, Beijing 100036;
    2. Institute of Toxicology and Pharmacology, Academy of Military Medical Science, Beijing100850
  • Received:1997-04-07 Revised:1998-03-03 Online:1998-09-15 Published:1998-09-15

Abstract: Objective: To study the effects of etimicin (EM) and gentamicin (GM) on renal endoplasmic reticulum 45Ca2+ uptake and Ca2+-Mg2+-ATPase activity. Methods: Using 45Ca2+ incorporation technique and peacock blue spectrophotometry respectively. Results: EM and GM (≥3.4×10-4 mol·L-1) inhibited endoplasmic reticulum 45Ca2+-uptake (the rate of inhibition: ≥17.4% and ≥25.5%, respectively); EM and GM (3.4×10-2 mol·L-1) inhibited Ca2+-Mg2+-ATPase activity (the rate of inhibition: 24.2% and 29.2%, respectively). Conclusion: At high concentration, EM and GM elevated intracellular calcium which may be related to their nephrotoxicity.

Key words: Etimicin (EM), Gentamicin (GM), Endoplasmic reticulum, 45Ca2+-uptake, Ca2+-Mg2+-ATPase

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