Determination of 2-Amino-6-Cyclopropylamino-9-(2,3-Dideoxy-β-D-glyceropent-2-enofuranosyl)purine in Rat Plasma, Urine and Liver Homogenates by High Performance Liquid Chromatography

Abstract

Abstract: Aim To develop a simple and specific high-performance liquid chromatographic (HPLC) method, suitable for the pharmacokinetic studies in Vivo, to determine the concentrations of 2-amino-6-cyclopropylamino-9-(2,3-dideoxy-β-D-glyceropent-2-enofuranosyl) purine (Cyclo-D4G, D4G prodrug) in rat plasma, urine and liver homogenates. Methods Chromatography was performed with C-18 Hypersil ODS column and a mobile phase of 7% (v/v) acetonitrile in phosphate buffer, pH7.40, with UV detection at 283 nm. Results The average extraction recovery of Cyclo-D4G in rat plasma and urine was 100.1% over its linear range of 0.5-80 μg·mL^-1. The accuracy of the assay was 99.4%. The intra-and interday RSDs were less than 9.0%. Conclusion The analytical method was found to be applicable, reliable and suitable for pharmacokinetic studies.

Key words: Pharmaceutical analysis, Pharmaceutical analysis, HPLC analysis, HPLC analysis, D4G prodrug, D4G prodrug, Stability, Stability

CLC Number:

R917

Supporting:

LIU Yi, YANG Zhen-jun^*, Boudinot F Douglas, CHU Chang kuang, ZHANG Li-he . Determination of 2-Amino-6-Cyclopropylamino-9-(2,3-Dideoxy-β-D-glyceropent-2-enofuranosyl)purine in Rat Plasma, Urine and Liver Homogenates by High Performance Liquid Chromatography[J]. .

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