Simultaneous Determination of Tetramethylpyrazine and Aspirin in a New Compound Formulation by Liquid Chromatography

Abstract

Abstract: Aim To establish a reversed-phase liquid chromatographic (LC) method for simultaneous determination of tetramethylpyrazine (TMP) and aspirin in a new compound formulation. Methods Chromatographic separation of the two drugs was achieved on a Diamonsil C18 column, using a binary mixture of methanol-1.5% acetic acid (35:65, V/V, pH = 3.1) as mobile phase at a flow rate of 1.0 mL·min^-1. Results Separation was completed in less than 12 min.Benzoic acid was used as the internal standard.Recoveries at levels corresponding to 80 % to 120 % of the label claim of the formulation ranged from 99.6 to 100.3 % for aspirin and from 99.9 to 101.3% for TMP.The linear range was 12.6-150.9 μg·mL^-1 (r = 0.9997, n = 5) for aspirin and 25.0-300.0 μg·mL^-1 (r = 0.9999, n = 5) for TMP. Conclusion The method developed can be used for the simultaneous determination of TMP and aspirin in pharmaceutical preparations.

Key words: liquid chromatography, liquid chromatography, tetramethylpyrazine, tetramethylpyrazine, asp irin, asp irin, assay, assay

CLC Number:

R917

R927.2

Supporting:

WANG Peng, QI Mei-ling^*, ZHOU Li, FANG Lin . Simultaneous Determination of Tetramethylpyrazine and Aspirin in a New Compound Formulation by Liquid Chromatography[J]. .

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