Proteomic study of Rehmannia glutinosa and Cornus officinalis herbal pair on the brain tissue of rats with ischemic stroke

doi:10.5246/jcps.2023.01.003

Abstract

Abstract:

Since the traditional Chinese medicine (TCM) "tonifying kidney and filling essence" treatment has an excellent therapeutic effect on ischemic stroke, Rehmannia glutinosa and Cornus officinalis were selected as the representative herbal combination to explore the effect of this therapeutic method on ischemic stroke. SD rats were used to establish the MCAO model and divided into two groups, including the model group and treatment group. Another sham operation group was set up, with 10 rats in each group. The treatment group was treated daily with Rehmannia Cornus Decoction by gavage, while the sham group and model group were treated with the same volume of distilled water by gavage. Brain tissue of the infarcted hemisphere was collected after 7 d of continuous intervention, then TTC staining was performed to evaluate the cerebral infarction volume, and the proteomic changes in the infarcted region of rats were detected by tandem mass tag spectrometry technology. The results showed that Rehmannia glutinosa and Cornus officinalis herb pair could effectively protect ischemic brain tissue and showed reliable proteomic assay quality and significant differences in proteomic expression profiles between the groups. A total of 993 proteins were differentially expressed between the treatment and model groups, of which 756 were up-regulated, and 237 were down-regulated. The functions of these differential proteins were mainly involved in regulating the activity of various enzymes and the changes in the binding functions of various molecules. The signaling pathways involved were mainly related to the synthesis and degradation of ketone, various synaptic functions, and phosphatidylinositol signaling system. These molecular functions and pathways might be the targets of this herbal pair for the intervention of ischemic stroke and provide a reference for further research.

Key words: Rehmannia glutinosa, Cornus officinalis, Ischemic stroke, Proteomics, Tonifying kidney and essence

Supporting:

Xin Li, Yan Shang, Mengxin Qi, Guoheng Hu. Proteomic study of Rehmannia glutinosa and Cornus officinalis herbal pair on the brain tissue of rats with ischemic stroke[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(1): 32-44.

Figures/Tables 5

References 19

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