Determination of chlorogenic acid and rutin in extractive solution of ficus virens leaves by HPLC-PDA

doi:10.5246/jcps.2023.01.004

Abstract

Abstract:

A HPLC-PDA method was established for determining chlorogenic acid (CGA) and rutin (RU) in the extract of Pueraria lobata leaves. The ODS2 column was used with gradient elution, the mobile phase was methanol–phosphoric acid (AR) solution (pH 3.0), the column temperature was 25 ± 2 oC, and the flow rate was 1.0 mL/min. The effective components in the extract were identified by mass spectrometry and UV spectrometry. The results showed that the extraction contained CGA and RU. The HPLC-PDA method for determining CGA and RU showed an excellent linear relationship within the concentration range, r ≥ 0.9998. The results showed that the limits of quantitation and detection were all less than 0.06 and 0.02 μg/mL, respectively. The relative recoveries ranged from 99.10% to 104.7%. The intra-day and inter-day precisions expressed by RSD values were all less than 1.89% and 1.82%, respectively. The method was sensitive, specific, and easy to operate and could be used to determine CGA and RU in the extract of Pueraria lobata leaves. The contents of RU and CGA were 3.46 and 3.23 μg/mL, respectively.

Key words: Ficus virens leaves, Chlorogenic acid, Extractive solution of F. virens var. sublanceolata, HPLC-PDA, Rutin

Supporting:

Yanqing Zhou, Yonghong Liu, Yanting Li, Yanru Tao, Jin Li, Xinhui Jiang. Determination of chlorogenic acid and rutin in extractive solution of ficus virens leaves by HPLC-PDA[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(1): 45-51.

Figures/Tables 4

References 22

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