HPLC-UV method for simultaneous determination of irbesartan, candesartan, gliquidone and pioglitazone in formulations and in human serum

doi:10.5246/jcps.2018.04.028

Abstract

Abstract:

In this study, we reported and validated a novel and sensitive reversed-phase liquid chromatographic method for the simultaneous determination of irbesartan, candesartan, gliquidone and pioglitazone. Separation was performed at 230 nm using a mobile phase consisting of methanol–water (90:10, v/v) with a flow rate of 1 mL/min. pH was adjusted to 3.5 with phosphoric acid. The concentration-response relationship was found linear over a concentration range of 5–25 μg/mL for all of the analytes tested. The limits of detection and quantification were 0.83 and 2.78 for irbesartan, 0.30 and 1.01 for candesartan, 1.11 and 3.93 for gliquidone, and 0.41 and 1.41 μg/mL for pioglitazone, respectively. Described method permitted the successful determination of these drugs in human serum. The developed method was simple, rapid, and it did not require extensive sample purification.

Key words: Candesartan, Gliquidone, Irbesartan, Pioglitazone, RP-HPLC, Method development

CLC Number:

R917

Supporting:

Agha Zeeshan Mirza. HPLC-UV method for simultaneous determination of irbesartan, candesartan, gliquidone and pioglitazone in formulations and in human serum[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(4): 273-280.

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