Simultaneous determination of two bioactive components of Huangqi Guizhi Wuwu Decoction in rat plasma using UPLC-MS/MS and its application to a pharmacokinetic study

doi:10.5246/jcps.2018.04.027

Abstract

Abstract:

A rapid, sensitive and selective UPLC-MS/MS method was developed to determine paeoniflorin and astragaloside IV. This method was validated via a pharmacokinetic study using rat plasma. The internal standard was clarithromycin. A simple one-step deproteinization procedure was used to prepare plasma samples. Separation was achieved on a CAPCELL CORE ADME C₁₈ column with a gradient mobile phase consisting of solution A (water containing 0.1% formic acid) and solution B (acetonitrile) at a flow rate of 0.3 mL/min. Multiple reaction monitoring (MRM) was used with an electrospray ionization source (ESI) in positive mode. A good linear response was observed within the ranges of 0.01 to 5.00 μg/mL for paeoniflorin and 0.0001 to 0.05 μg/mL for astragaloside IV. The accuracy (RE) was within the range of –3.5% to 6.3%, and the intra- and inter-day precisions (RSD) were within 14.2%. The extraction recoveries were all above 78.9%. The pharmacokinetic study of the two analytes in rats after oral administration of Huangqi Guizhi Wuwu Decoction (HGWD) was successfully completed through this method. The method developed in this study will fill a gap in pharmacokinetic studies of HGWD.

Key words: Huangqi Guizhi Wuwu Decoction, Pharmacokinetics, Rat plasma, UPLC-MS/MS

CLC Number:

R969.1

Supporting:

Xinyu Chang, Guiming Guo, Zheng Fan, Honglei Wang, Yang Liu, Lijuan Han. Simultaneous determination of two bioactive components of Huangqi Guizhi Wuwu Decoction in rat plasma using UPLC-MS/MS and its application to a pharmacokinetic study[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(4): 263-272.

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