Qualitative analysis of the chemical constituents of Lonicera japonica and its tetraploid cultivar Jiufengyihao

doi:10.5246/jcps.2015.12.103

Abstract

Abstract:

In the present study, we, for the first time, developed an UPLC-IT-TOF/MS method to compare the chemical constituentsof the flower buds of Lonicera japonicaThunb. (Jinyinhua, JYH, in Chinese) and its tetraploid cultivar Jiufengyihao (JFYH). The samples were separated on a C₁₈ ultra-column (100 mm×2.1 mm, 1.8 μm) eluted with a mixture of acetonitrile and 0.1% formic acid using a gradient program. Based on the accurate masses, fragmental MS² ions and fragmentation pathway, we unambiguously affirmed or tentatively identified 30 compounds, including 11 phenolic acids, 10 iridoid glycosides, 8 flavonoids and 1 saponin, from JYH and JFYH. Among them, all compounds were detected in Lonicera japonica, but only 21 compounds were detected in JFYH. In addition, the relative contents of the 21 common chemical constituents were also different in JYH and JFYH, suggesting an obvious chemical difference between JYH and JFYH.

Key words: UPLC-IT-TOF/MS, Lonicera japonica, Jiufengyihao, Tetraploid cultivar

CLC Number:

R284

Supporting:

Xiao Zhang, Qing Guo, BoyangYu, Ming Li, Lixia Huo, Junjun Wang. Qualitative analysis of the chemical constituents of Lonicera japonica and its tetraploid cultivar Jiufengyihao[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(12): 809-815.

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