Bioconversion of genistein to (-)-5-hydroxy-equol by a newly isolated cock intestinal anaerobic bacterium

doi:10.5246/jcps.2015.07.057

Abstract

Abstract:

A newly isolated bacterium, named as AUH-JLC257, was found to be capable of bioconverting isoflavone genistein to 5-hydroxy-equol under anaerobic conditions. The metabolite 5-hydroxy-equol was identified by using UV spectrum, electrospray ionization mass spectrometry (ESI-MS) as well as ¹H and ¹³C NMR analyses. Chiral stationary-phase high-performance liquid chromatography analysis and specific rotation examination demonstrated that the bio-synthesized 5-hydroxy-equol was just (–)-5-hydroxy-equol. The average bioconverting rate was 83.1%, and the strain AUH-JLC257could efficiently transform genisteinat a maximal substrate concentration of 0.6 mmol/L. We, for the first time, showed that the bio-synthesized 5-hydroxy-equol had 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity at concentrations as low as 3.3 µmol/L.In addition, the 16S rRNA gene sequence (1401 bp) of the bacterium strain AUH-JLC257 showed the highest similarity (99.27%) to that ofSlackia equolifaciens strain DZE.

Key words: Genistein, 5-Hydroxy-equol, Bacterial isolation, Identification, Microbial biotransformation

CLC Number:

R284

Supporting:

Yanjing Xie, Ziguang Liu, Yaning Gao, Xiuling Wang, Qinghong Hao, Xiumei Yu. Bioconversion of genistein to (-)-5-hydroxy-equol by a newly isolated cock intestinal anaerobic bacterium[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(7): 442-448.

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