The involvement of signaling activation of protein kinase C in gadolinium chloride-induced cell survival and cell cycle progression in NIH3T3 cells

doi:10.5246/jcps.2014.11.098

Abstract

Abstract:

In the present study, we investigated the activation of protein kinase C (PKC) family in mouse embryonic fibroblast NIH3T3 cells using gadolinium chloride as a representative lanthanide ion. With live cell imaging system and confocal laser scanning microscopy, we found that the treatment of 50 μM GdCl₃promoted cell survival under the condition of serum-starvation. Moreover, better cell attachment and cytoskeleton reorganization were also observed. Additionally, GdCl₃treatment resulted in the phosphorylation of PKC family at different time points. Furthermore, bisindolylmaleimide (a PKCpan inhibitor) could efficiently reduce the level of phosphorylated PKCpan (βII^Ser660), alleviating ERK activation induced by GdCl₃. This finding indicated that the PKC activation was involved in GdCl₃-induced MAPK/ERK signaling and thus might contribute to GdCl₃-induced cell cycle progression and cell survival.

Key words: Gadolinium chloride, Cell cycle, Protein kinase C, Cytoskeleton, ERK, NIH3T3 cells

CLC Number:

R915

Supporting:

Min Feng, Jinxia Li, Xiaojie Ma,Yunzhou Fan, Jingxuan Wu, Qun Zeng, Xiaogai Yang. The involvement of signaling activation of protein kinase C in gadolinium chloride-induced cell survival and cell cycle progression in NIH3T3 cells[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(11): 772-777.

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